TY - JOUR T1 - Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum AU - Hossain, Chowdhury Faiz AU - Al-amin, Mohammad AU - Siddiqui, Mohammad Arafat AU - Ruma, Shamima Afrin AU - Eltayeb, Nagla Mustafa AU - Sultana, Gazi Nurun Nahar AU - Muhamad Salhimi, Salizawati PY - 2025 DA - June JF - Journal of Research in Pharmacy JO - J. Res. Pharm. PB - Marmara University WT - DergiPark SN - 2630-6344 SP - 559 EP - 566 VL - 23 IS - 3 LA - en AB - The rhizome of Zingiber roseum has been used as traditional medicines for the treatment of gastric ulcer, skin diseases, and liver infections. Zerumbone is an essential sesquiterpenoid previously isolated from different species of Zingiberaceae and possesses numerous pharmacological properties. Based on previous reports, the aim of the current study was to investigate the antimicrobial activity of extract, fractions, and isolation of zerumbone from Zingiber roseum. The antimicrobial activity was determined using disc diffusion method. The serial tube dilution technique was used to determine Minimum Inhibitory Concentration (MIC) of the samples. Vacuum Liquid Chromatography (VLC) and open column chromatography were used for separation. The methanol extract exhibited maximum antimicrobial activity against Escherichia coli, Saccharomyces cerevisiae, and Aspergillus niger in both methods. VLC of the extract yielded five fractions (Fr. 1 to Fr. 5). Fr. 1 exhibited maximum antimicrobial activity compared to the other fractions. Column chromatography of Fr.1 generated 47 fractions. Fraction 1 to 9 were combined and crystallized in n-hexane to isolate zerumbone as the most abundant source (yield 6.5 % from dried MeOH extract). Zerumbone showed good activity against all tested bacteria. The results of our study might validate the ethnobotanical use of Zingiber roseum rhizomes in the treatment of infectious diseases. Moreover, Zingiber roseum could be one of the most abundant sources for the isolation of natural zerumbone to study different pharmacological studies in the field of new drug discovery. KW - Zingiber roseum KW - antimicrobial activity KW - MeOH extract KW - VLC fractions KW - zerumbone. CR - [1] Hossain CF, Al-Amin M, Rahman KMM, Sarker A, Alam MM, Chowdhury MH, Khan SN, Sultana GNN. Analgesic principle from Curcuma amada. J Ethnopharmacol. 2015; 163: 273–277. [CrossRef] CR - [2] Hossain CF, Al-Amin M, Sayem ASM, Siragee IH, Tunan AM, Hassan F, Kabir MM, Sultana GNN. Antinociceptive principle from Curcuma aeruginosa. BMC Complement Altern Med. 2015; 15: 191. [CrossRef] CR - [3] Ganesan S, Pandi NR, Banumathy N. Ethnomedicinal survey of Alagarkoil Hills (Reserved forest), Tamil Nadu, India. eJ Indian Med. 2007; 1: 1-18. CR - [4] Padal SB, Ramakrishna H, Devender R. Ethnomedicinal studies for endemic diseases by the tribes of Munchingiputtu Mandal, Visakhapatnam district, Andhra Pradesh, India. Int J Med Arom Plants 2012; 2: 453-459. CR - [5] Prakash O, Kasana VK, Pant AK, Zafar A, Hore SK, Mathela CS. Phytochemical composition of essential oil from seeds of Zingiber roseum Rosc. and its antispasmodic activity in rat duodenum. J Ethnopharmacol. 2006; 106: 344-347. [CrossRef] CR - [6] Prakash O, Pant AK. Linalool-rich essential oil from the rhizomes of Zingiber roseum Rosc. J Essent Oil Res. 2006; 18: 638-639. [CrossRef] CR - [7] Ram AJ, Bhakshu LM, Raju RRV. In vitro antimicrobial activity of certain medicinal plants from Eastern Ghats, India, used for skin diseases. J Ethnopharmacol. 2004; 90: 353-357. [CrossRef] CR - [8] Matthes HWD, Luu B, Ourisson G. Cytotoxic components of Zingiber zerumbet, Curcuma zedoaria and C. domestica. Phytochemistry 1980; 19: 2643-2650. [CrossRef] CR - [9] Ogunwande IA, Olawore NO, Adeleke KA. Contribution to the study of essential oil of Xylopia aethiopica (Dunal) A. Rich: Isolation and characterization of zerumbone. J Essent Oil Bear Pl 2005; 8: 159-164. [CrossRef] CR - [10] Kirana C, McIntosh GH, Record IR, Jones GP. Antitumor activity of extract of Zingiber aromaticum and its bioactive sesquiterpenoid zerumbone. Nutr Cancer 2003; 45: 218-225. [CrossRef] CR - [11] Al-Amin M, Sultana GNN, Hossain CF. Antiulcer principle from Zingiber montanum. J Ethnopharmacol. 2012; 141: 57–60. [CrossRef] CR - [12] Sulaiman MR, Perimal EK, Zakaria ZA, Mokhtar F, Akhtar MN, Lajis NH, Israf DA. Preliminary analysis of the antinociceptive activity of zerumbone. Fitoterapia 2009; 80: 230-232. [CrossRef] CR - [13] Sulaiman MR, Perimal EK, Akhtar MN, Mohamad AS, Khalid MH, Tasrip NA, Mokhtar F, Zakaria ZA, Lajis NH, Israf DA. Anti-inflammatory effect of zerumbone on acute and chronic inflammation models in mice. Fitoterapia 2010; 81: 855-258. [CrossRef] CR - [14] Dai JR, Cardellina II JH, Mahon JBM, Boyd MR. Zerumbone, an HIV inhibitory and cytotoxic sesquiterpene of Zingiber aromaticum and Z. zerumbet. Nat Prod Lett. 1997; 10: 115-118. [CrossRef] CR - [15] Sithara T, Dhanya BP, Arun KB, Sini S, Dan M, Vasu RK, Nisha P. Zerumbone, a cyclic sesquiterpene from Zingiber zerumbet induces apoptosis, cell cycle arrest, and antimigratory effects in SW 480 colorectal cancer cells. J Agric Food Chem. 2018; 66: 602-612. [CrossRef] CR - [16] Sung B, Prasad S, Yadav VR, Aggarwal BB. Cancer cell signaling pathways targeted by spice-derived nutraceuticals. Nutr Cancer 2012; 64: 173-197. [CrossRef] CR - [17] Duru ME, Öztürk M, Ugur A, Ceylan O. The constituents of essential oil and in vitro antimicrobial activity of Micromeria cilicica from Turkey. J Ethnopharmacol. 2004; 94: 43–48. [CrossRef] CR - [18] Khan A, Rahman M, Islam MS. Antibacterial, antifungal and cytotoxic activities of salviasperanol isolated from Amorphophallus campanulatus. Pharm Biol. 2009; 47: 1187–1191. [CrossRef] CR - [19] Kuete V, Nana F, Ngameni B, Mbaveng AT, Keumedjio F, Ngadjui BT. Antimicrobial activity of the crude extract, fractions and compounds from stem bark of Ficus ovata (Moraceae). J Ethnopharmacol. 2009; 124: 556–561. [CrossRef] UR - https://dergipark.org.tr/en/pub/jrespharm/issue//1691376 L1 - https://dergipark.org.tr/en/download/article-file/4836603 ER -