@article{article_1703748, title={INVESTIGATION OF THE ANTIPROLIFERATIVE EFFECTS OF THIOCOLCHICOSIDE ON A549 LUNG CANCER CELLS}, journal={Current Research in Health Sciences}, volume={2}, pages={61–66}, year={2025}, DOI={10.62425/crihs.1703748}, author={Atasever, Seher and Aydın, Aylin and Uğan, Rüstem Anıl}, keywords={Thiocolchicoside, A549, wound healing, antiproliferative}, abstract={Objective: This study aims to determine the IC₅₀ value of Thiocolchicoside on A549 human non-small cell lung cancer cells using the MTT assay and to investigate its antiproliferative and wound healing effects.
Methods: The cytotoxic and wound healing effects of Thiocolchicoside on A549 cells were evaluated in vitro. Cell viability was assessed at 24, 48, and 72 hours by MTT assay to calculate IC₅₀ values. Additionally, an in vitro wound healing (scratch) assay was performed to evaluate cell migration.
Results: The IC₅₀ value of Thiocolchicoside at 24 hours was found to be 2.693×10⁻⁴ M. MTT assay results showed significant cytotoxicity at high concentrations, while lower concentrations had no significant effect or increased cell viability. Cytotoxic effects increased over time, being more evident at 48 and 72 hours. In wound healing assays, low concentrations of Thiocolchicoside significantly enhanced wound closure, whereas higher concentrations reduced this effect. Statistical analysis revealed significant differences between concentrations at 24 and 48 hours, except for the lowest concentration at 24 hours.
Conclusion: Thiocolchicoside exerts dose- and time-dependent cytotoxic effects on A549 cells and may promote wound healing at low concentrations, suggesting potential dual roles in cancer treatment and tissue repair.}, number={2}, publisher={Ataturk University}, organization={Scientific and Technological Research Council of Turkey (TUBITAK) 2209A}