@article{article_1704479, title={Effects of antioxidants used in the experimental premature ovarian insufficiency model on mitophagy proteins and them-associated MicroRNAs}, journal={Ege Tıp Dergisi}, volume={64}, pages={688–700}, year={2025}, DOI={10.19161/etd.1704479}, url={https://izlik.org/JA77PY77RT}, author={Uçar Kartal, Duygu and Kuşçu, Naci Kemal and Özbilgin, Kemal and Pekmezci, Muhammed Yusuf}, keywords={Cyclophosphamide, Curcumin, Quercetin, MiRNA, POI, Parkin, FUNDC1}, abstract={Aim: It was aimed to investigate the protective and therapeutic effects of quercetin and curcumin in POI by examining follicle count, immunohistochemical proteins, mRNAs and their related microRNAs. Materials and Methods: In our study, an experimental premature ovarian insufficiency model was created on rats by means of cyclophosphamide. Then the tissues were examined by histopathological methods and histochemical staining. We studied the changes in parkin-dependent and independent mechanisms that occur with quercetin and curcumin. RNA isolation was performed on ovarian samples and cDNA synthesis, and qPCR processes were performed. The difference between the obtained findings and the groups was evaluated statistically. Results: Cyclophosphamide depleted primordial follicles, which is the main pool for fertility, on ovarian tissue and induced atresia (p <0.001). Curcumin and quercetin acted on parkin-dependent pathways, resulting in significant improvement in the ovarian follicle pool (p <0.001). Conclusion: The significant increase in the primordial follicle pool and the decrease in the rate of atretic follicles observed in the groups given curcumin and quercetin indicate that curcumin and quercetin have protective and therapeutic roles in the pathogenesis of POI by regulating mitophagy in the parkin-mediated pathway on the ovary.}, number={4}