TY  - JOUR
T1  - NADPH oxidase-2 inhibitor apocynin attenuates high-fat diet-induced kidney and bladder injury
AU  - Ercan, Feriha
AU  - Kanpalta Mustafaoğlu, Fatma
AU  - Ertaş, Büşra
AU  - Şener, Göksel
PY  - 2025
DA  - May
Y2  - 2025
DO  - 10.5472/marumj.1706664
JF  - Marmara Medical Journal
JO  - Marmara Med J
PB  - Marmara University
WT  - DergiPark
SN  - 1019-1941
SP  - 167
EP  - 178
VL  - 38
IS  - 2
LA  - en
AB  - Objective: This study aimed to evaluate the effects of NADPH oxidase-2 (NOX-2) inhibitor apocynin (APC) on high-fat diet (HFD)-induced renal and bladder injury.Materials and Methods: Wistar albino rats were divided into 4 groups: Control, HFD, HFD+dimetyl sulfoxide (DMSO), and HFD+APC.Rats in HFD, HFD+DMSO, and HFD+APC groups were fed with HFD for sixteen weeks. In the last 4 weeks of the experiment, eitherDMSO or APC (25 mg/kg, dissolved in DMSO) was applied to the HFD+DMSO or HFD+APC groups. Lipid profiles and leptinvalues were measured in blood serum. Renal and bladder oxidant/antioxidant parameters, histological changes in the tissues, NOX-2-,nuclear factor kappa B (NF-ĸB)-immunopositive and apoptotic cells were evaluated.Results: At the end of the experiment, leptin, cholesterol, and triglyceride levels were higher and high-density lipoprotein levels werelower in the HFD and HFD+DMSO groups compared to controls. In these experimental groups, an increase in malondialdehyde,8-hydroxy-deoxyguanosine and myeloperoxidase levels and a decrease in glutathione levels, as well as an increase in collagen, NOX-2– and NFĸ-B-immunopositive and apoptotic cells were found. Also, a deterioration in kidney and bladder morphology was observed.All these biochemical and histopathological findings improved in the HFD+APC group.Conclusion: High-fed diet causes renal and bladder injury by increasing NOX-2 activity and inflammation via oxidative stress. APCmight alleviate tissue injury by inhibiting oxidative stress.
KW  - Apocynin
KW  - Bladder
KW  - High-fat diet
KW  - Kidney
KW  - Oxidative stress
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UR  - https://doi.org/10.5472/marumj.1706664
L1  - https://dergipark.org.tr/en/download/article-file/4903371
ER  -