TY - JOUR T1 - Effect of Different Supplements on Ensiling Properties, In Vitro Digestibility and Energy Content of Lenox (Brassica rapa L.) AU - Kale, Çağrı AU - Öztürk, Mehmet Uğur AU - Deniz, Suphi AU - Özkan, Fatma PY - 2025 DA - September Y2 - 2025 DO - 10.31015/2025.3.32 JF - International Journal of Agriculture Environment and Food Sciences JO - int. j. agric. environ. food sci. PB - Gültekin ÖZDEMİR WT - DergiPark SN - 2618-5946 SP - 923 EP - 929 VL - 9 IS - 3 LA - en AB - The aim of this experiment was to investigate the effects of wilting and ensiling Lenox plants with different additives on nutrient contents, silage quality, in vitro digestibility, and energy contents. In the study, 5 groups were formed: 100% Lenox (L), 100% wilted Lenox (WL), 95% Lenox + 5% molasses (LM), 96% Lenox + 4% grinded wheat (LW), and 70% Lenox + 30% ryegrass (LR). Thirty silage samples, prepared as 6 replicates in each group, were filled into 1-liter jars. Silages were incubated for 60 days. Wilting and addition of molasses, grinded wheat or ryegrass increased the dry matter content of the silages. The addition of molasses or grinded wheat increased the crude protein content of the silages. While the NDF and ADF values were similar in the L and WL groups, the NDF and ADF values of the other groups were lower than those of the L (P<0.001). All silage pH values determined in the study were within the range of 3.8-4.2 predicted for quality silages. The Fleig score of all silages was classified as "good" quality. The highest lactic acid value was recorded in the LR, followed by the WL, LM, L and LW respectively. Acetic acid content was determined to be quite low in all groups. The highest value of NH3-N content was obtained in LW. Various additives added to lenox increased the in vitro dry matter and organic matter digestibility of the silages. This effect was also reflected in the digestibility energy, metabolic energy and net energy for lactation levels of the silages. As a result; lenox, when harvested at full flowering, can be ensiled with pure or easily digestible carbohydrate-rich additives; however, it was found that the additives increased the evaluability of the silages. KW - Additive KW - Energy content KW - In vitro digestibility KW - Lenox silage CR - Ankom. (2002). Operator’s Manual ANKOM ll 200/220 Fiber Analyzer. ANKOM Technology Corp., Fairport, NY. CR - AOAC. (1990). Official Method of Analysis. 15th ed. Association of Official Analytical Chemists, Washington, DC. CR - Bacaksiz, O.K. (2024). The Effect Of Tannin Addition to Turnip Forage Silage (Lenox/Brassica Rapa L.) on Silage Fermentation and In Vitro Digestibility. Balıkesir University, Balıkesir. CR - Baytok, E., Aksu, T., Karsli, M.A., Muruz, H. (2005). The Effects of Formic Acid, Molasses and Inoculant as Silage Additives on Corn Silage Composition and Ruminal Fermentation Characteristics in Sheep. Turkish Journal of Veterinary & Animal Sciences, 29, 469-474. https://journals.tubitak.gov.tr/veterinary. CR - Bingol, N.T., Karsli, M.A., Bolat, D., Akca, I. (2008). The Effects of Molasses and Formic Acid Addition in Sainfoin Silage Harvested at Different Maturities on Silage Quality and Digestibility. Van Veterinary Journal,19, 61-66. CR - Bolsen, K.K., Ashbell, G., Weinberg, Z.G. (1996). Silage Fermentation and Silage Additives. Asian-Australasian Journal of Animal Sciences, 9, 483-494. CR - Carpemtero, M.C., Holding, A.C., Mcdonald, P. (1969). Fermentation studies on lucerne. Journal of the Science of Food and Agriculture, 20, 677-681. https://doi.org/10.1002/jsfa.2740201112. CR - Dogan Das, B. (2019). The Effect of Different Levels of Wheat Straw and Dried Molasses Sugar Beet Pulp on The Silage Quality, Live Weight Gain in Lambs and Digestibility of Lenox (Brassica rapa L.) Silage. Harran University, Urfa. CR - Ergun, A., Tuncer, S.D., Colpan, I., Yalcın, S., Yildiz, G., Kucukersan, M.K., Kucukersan, S., Sehu, A., Sacakli, P. (2013). Feeds, Feed Hygiene and Technology. Ankara, Turkey, Pozitif, 5. press, 440 pp. CR - Gemalmaz, E., Bilal, T. (2016). Alternative Roughage Sources. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 56, 63-69. CR - Keady, T.W.J. (1996). A Review of the Effects of Molasses Treatment of Unwilted Grass at Ensiling on Silage Fermentation, Digestibility and Intake and on Animal Performance. Irish Journal of Agricultural and Food Research, 35, 141. https://www.jstor.org/stable/25562278. CR - Kilic, A. (1986). Silo Feed (Teaching, Learning and Application Suggestions). İzmir, Turkey, Bilgehan press, 327 pp. CR - Kilic, U., Erisek, A. (2019). Effects of Additive Use on Silage Quality and In vitro Digestibility of Some Brassica silages. Journal of Scientific and Engineering Research, 6, 163-171. CR - Kirchgebner, M. (1987). Tierernahrung, DLG-Verlag- Frankfurt. CR - Kung, L.J.R. (2019). Aerobic stability of silage. Available: http://alfalfa.ucdavis.edu/+symposium/ proceedings/2010/10-89.pdf. Proceedings California: Alfalfa and Forage Symposium. Assessed on Erişim tarihi: September 20, 2019. CR - Leventini, M.W., Hunt, C., Roffler, R.E., Casebolt, D.G. (1990). Effect of dietary level of barleybased supplements and ruminal buffer on digestion and growth by beef cattle. Journal of Animal Science,68, 4334-4344. https://doi.org/10.2527/1990.68124334x. CR - MAFF. (1975). Energy Allowances and Feeding Systems for Ruminants. Ministery of Agriculture, Fisheries and Food. Department of Agriculture and Fisheries for Scotland, Department of Agriculture for Northern Ireland. Her Majesty’s Stationary Office. London. Markham, P. (1942). A steam distilation apparatus suitable for micro-kjeldahl analyses. The Journal of Biochemistry, 36, 790-797. https://doi.org/10.1042/bj0360790. CR - Menke, K.H., Huss, W. (1987). Tierernahrung. Verlag Eugen Ulmer Stuttgart. CR - Osbourn, D.F. (1987). Principles governinig the use of chemical methods for assessing the nutritive value of forages: A Review. Animal Feed Science and Technology, 3, 265-275. https://doi.org/10.1016/0377-8401(78)90001-9. CR - Ogretmen, T. (1991). Determination of NEL content of some important feeds used in ruminant nutrition by in vivo and in vitro methods. Ege University, İzmir. CR - Ozkan, F. (2019). Determining the Quality of Sugar Beet Pulp, Lenox and Ryegrass Silages Used in Feeding Dairy Cattle, in Comparison with Corn Silage.Van Yuzuncu Yil University, Van. CR - Ozkan, F., Deniz, S. (2023). Determining the quality of sugar beet pulp, lenox, and ryegrass silages used in feeding dairy cattle, in comparison to corn silage. Turkish Journal of Veterinary & Animal Sciences, 47, 565-575. https://doi.org/10.55730/1300-0128.4326. CR - Ozkul, H., Kirkpinar, F., Tan, K. (2012). Utilization of Caramba (Lolium Multiflorum cv. Caramba) Herbage in Ruminant Nutrition. Journal of Animal Production, 53, 21-26. CR - Parlak, A.O., Sevimay, C.S. (2007). Possibilities of Growing Some Forage Plants as Second Crop After Barley and Wheat Harvest. Journal of Agricultural Sciences, 13, 101-107. CR - Polan, C.E., Stive, D.E., Garrett, J.L. (1998). Protein preservation and ruminal degradation of ensiled forage treated with heat, formic acid, ammonia, or microbial inoculant. Journal of Dairy Science, 81, 765-776. https://doi.org/10.3168/jds.S0022-0302(98)75633-4. CR - Rao, S.C., Horn, F.P. (1995). Cereals and Brassicas for Forage, The Science of Grassland Agriculture, Ames: 5th ed., Iowa State Univ. Press, 451- 462 pp. CR - Sandrin, C.Z., Domingos, M., Figueiredo-Ribeiro, R.C.L. (2006). Partitioning of water soluble carbohydrates in vegetative tissues of Lolium multiflorum Lam. ssp. İtalicum cv. Lema. Braz. Journal of Plant Physiology, 18, 299-305. CR - SPPS. (2006). IBM SPSS statistics version 13.0 for Windows. New York: IBM Corp. CR - Stefanie J.W.H., Elferink H.O., Driehuis F., Gottschal J.C., Spoelstra S.F. (1999). Silage Fermentation Processes and Their Manipulation. FAO Electronic Conference on Silages;1-28. CR - Tiknazoglu, B. (2006). Forage crops agriculture and silage making. Available: https://kutuphane.tarimorman.gov.tr/vufind/MyResearch/UserLogin. Assessed on July 20, 2022. CR - Tobioka, H., Pradhan, R., Tasaki, I. (1991). The Effects of Various Additives on the Digestibility and İntakes of Whole-Crop Barley Silages by Wether. Proceedings of Faculty of Agriculture, Kyushu Tokai University; Japan. CR - Van ES, A.J.H. (1978). Feed evaluation for ruminants. I. The systems in use from May 1977 onwards in The Netherlands. Livestock Production Science,5, 331-345. https://doi.org/10.1016/0301-6226(78)90029-5. CR - Van Soest, P.J. (1982). Analytical systems for evaluation of feeds. Nutritional Ecology of The Ruminant. In: Van Soest PJ. (Editor). Cornell University Press, Chapter 6:75-94. CR - Virtanen, A.I. (1933). The AIV method of preserving fresh fodder. Empire Journal of Experimental Agriculture,1, 143-155. CR - Ward, R.T., De Ondarza, M.B. (2008). Effect of month of sample submittal on corn silage nutrient fractions starch availability, ndf digestibility, and fermentation profiles measured at a commercial forage-testing laboratory. Journal of Dairy Science, 91, 30. UR - https://doi.org/10.31015/2025.3.32 L1 - https://dergipark.org.tr/en/download/article-file/4909206 ER -