TY  - JOUR
T1  - T Regulatory Cells in Children with Atopic Dermatitis
TT  - Atopik Dermatitli Çocuklarda Regülatör T Hücreleri
AU  - Haskoloğlu, Zehra  Şule
PY  - 2018
DA  - October
DO  - 10.4274/atfm.87597
JF  - Ankara Üniversitesi Tıp Fakültesi Mecmuası
PB  - Ankara University
WT  - DergiPark
SN  - 0365-8104
SP  - 118
EP  - 122
VL  - 71
IS  - 2
LA  - en
AB  - Objectives: The aim of this study was to examine peripheral blood CD4+CD25high+FOXP3+ regulatory T (Treg) cell levels in children with atopic dermatitis (AD) and to evaluate their effects on the pathogenesis of the disease.Materials and Methods: Forty one patients (age between 1-60 months) satisfied the criteria for AD (Hanifin and Rajka criteria) and 20 healthy children were included into the study. Intracytoplasmic FOXP3 expression in CD4+CD25+ cells was measured by flow cytometry (Beckman Coulter FC500, USA, Software CXP 1.2). The change in mean fluorescence intensity (MFI) of the FOXP3 molecule relative to the MFI of the isotypic control was based on evaluation.Results: Children with atopic dermatitis have significantly lower levels of peripheral blood CD4+CD25high+FOXP3+ regulatory T cells and higher levels of CD4+CD25high+FOXP3+ regulatory T cell MFI than the control group (p
KW  - Atopic Dermatitis
KW  - Regulatory T
KW  - FOXP3 Expression
KW  - Flow Cytometry
KW  - Mean Fluorescence Intensity Levels
N2  - Amaç: Bu çalışmanın amacı, atopik dermatitli (AD) çocuklarda periferik kanda CD4+CD25high+FOXP3+ regülatör T (Treg) hücreleri ve bu hücrelerin hastalığın patogenezi üzerine etkilerini değerlendirmektir.Gereç ve Yöntem: AD kriterlerini (Hanifin ve Rajka kriterlerini) karşılayan 41 AD’li hasta (yaş aralığı: 1-60 ay) ve 20 sağlıklı çocuk çalışmaya dahil edildi. CD4+CD25 + hücrelerde intrasitoplazmik FOXP3 ekspresyonu akım sitometrisi (Beckman Coulter FC500, USA, Software CXP 1.2) ile ölçüldü. Değerlendirmede FOXP3 molekülünün ortalama floresan yoğunluğunun (MFI) izotipik kontrolün MFI’ya göre değişimi esas alındı.Bulgular: AD’li çocukların kontrol grubuna göre; periferik kan Treg hücre seviyeleri düşük ve CD4+CD25high+FOXP3+Treg hücre MFI’ı daha yüksek bulundu (p
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UR  - https://doi.org/10.4274/atfm.87597
L1  - https://dergipark.org.tr/en/download/article-file/4945491
ER  -