@article{article_1717548, title={Screening of Aerobic Spore-Forming Soil Bacteria for Potential Biotechnological Applications}, journal={Kastamonu University Journal of Engineering and Sciences}, volume={11}, pages={47–56}, year={2025}, DOI={10.55385/kastamonujes.1717548}, author={Albayrak İskender, Nurcan}, keywords={Aerobic Spore-Forming Bacteria, Enzymatic Activity, Bacteriocin, Antibacterial Effect}, abstract={Soils represent a significant source of bacteria that produce enzymes and bioactive compounds; however, many microorganisms with high biotechnological potential remain insufficiently explored for isolation. The aim of this study was to isolate aerobic spore-forming bacteria from soil and to identify potential strains for biotechnological applications. Soil samples were collected from various locations in Artvin province, where olive trees are present. The morphological and biochemical characteristics of 11 bacterial isolates were evaluated, and their molecular identification was performed through 16S rRNA gene sequencing. The isolates were identified as belonging to Priestia megaterium, Bacillus amyloliquefaciens, and Bacillus subtilis species. All isolates were screened for protease, amylase, and cellulase production, with five isolates demonstrating the ability to produce all three enzymes simultaneously. Additionally, the bacteriocin production capacities of the isolates were qualitatively assessed, revealing that several isolates significantly inhibited the growth of other strains. The resistance of the isolates to UV radiation was also evaluated; while short-term UV exposure caused a reduction in growth for some isolates, most exhibited high tolerance. The obtained results indicate that the bacterial isolates possess promising potential for various industrial and biotechnological applications due to their multi-enzyme production, antibacterial activities, and resilience to environmental stressors. Furthermore, the potential of these isolates in other biotechnological fields has been discussed in light of the current literature.}, number={1}, publisher={Kastamonu University}