TY - JOUR T1 - Comparison of various columns used in anion exchange chromatography method for mAb purification AU - Uslu, Mehmet Emin AU - Ozturk, Fatih PY - 2025 DA - August Y2 - 2025 DO - 10.38042/biotechstudies.1733671 JF - Biotech Studies JO - Biotech Studies PB - Tarla Bitkileri Merkez Araştırma Enstitüsü WT - DergiPark SN - 2687-3761 SP - 73 EP - 82 VL - 34 IS - SI LA - en AB - The impact of resins with varying ligands and pH levels on human IgG4 protein was analysed using anion exchange chromatography. Initially, a resin screening study involving five different positively charged ligands from four different brands was conducted on largely purified monoclonal antibodies, following Protein A capture. Subsequently, the influence of pH levels (6, 7, and 8.1) on an IgG4 protein with an isoelectric point of 6.9 was assessed using a single resin. Throughout the resin screening, all protein quality analyses were performed to identify the resin with the most compatible ligand. 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