TY - JOUR T1 - Comparative studies on anti-inflammatory and antidiabetic effects of some Achillea species from the Turkish flora AU - Bitiş, Leyla AU - Gürbüzkol, Fatmanur PY - 2025 DA - July Y2 - 2024 DO - 10.12991/jrespharm.1734601 JF - Journal of Research in Pharmacy JO - J. Res. Pharm. PB - Marmara University WT - DergiPark SN - 2630-6344 SP - 1601 EP - 1607 VL - 29 IS - 4 LA - en AB - This study aimed to determine antioxidant, antidiabetic and anti-inflammatory activities of methanolextracts of Achillea crithmifolia Waldst. & Kit. (AC), A. millefolium L. (AM), A. nobilis subsp. neilreichii (A.Kern.) Velen.(AN), A. setacea Waldst. & Kit. (AS) and A. wilhelmsii K. Koch (AW) species which grow naturally in Türkiye.DPPH/ABTS radicals scavenging, α-glucosidase inhibition and anti 5-lipoxygenase methods were used to determinefor antioxidant, antidiabetic and anti-inflammatory activities, respectively. The total phenol content of the extracts wascalculated using Folin-Ciocalteau solution and the highest phenol content was found in AC methanol extract (ACM)(56.71 mg GAE/g). In DPPH and ABTS radical scavenging activity assays, ACM showed the highest antioxidant activityaccording to IC50 values (54.85 and 79.77 μg/mL, respectively). According to the results of α-glucosidase inhibitoryactivity test, AN methanol extract (ANM) with an IC50 value of 245.50 μg/mL showed a better activity than acarbose(261.7 μg/mL) by inhibiting the enzyme. As a result of anti-inflammatory activity, it was observed that ACM had thehighest activity (90.30 μg/mL). Therefore, ACM, which has the best antioxidant, anti 5-lipoxygenase activity and thesecond best value close to the standard in terms of α-glucosidase inhibitory activity, was fractionated with 3 differentsolvents (hexane-ACH, chloroform-ACC and ethyl acetate-ACEA). Biological activity studies were repeated on thesefractions. The ACH fraction showed the best anti-inflammatory activity while the ACEA fraction showed the bestantidiabetic activity. The biological activities of five Achillea species were not examined before by comparing with eachother. In addition, antidiabetic and anti-inflammatory activity studies on A. crithmifolia were conducted for the firsttime. KW - Achillea crithmifolia KW - Anti 5-lipoxygenase KW - α-glucosidase inhibition KW - Anti-inflammatory KW - Antidiabetic CR - Semiz G, Gunal B, Dogan NM. Chemical composition and anti-biofilm activity of the essential oil from Achillea alimeana. Chemistry of Natural Compounds. 2023; 59: 984-986. https://doi.org/10.1007/s10600-023-04173-7 CR - Amanpour A, Çelebi F, Kahraman IG, Çelik F. Diyet inflamatuar indeksi, inflamasyon ve beslenme. Sağlık Bilimleri ve Araştırmaları Dergisi. 2022; 5(3): 59-80. https://doi.org/10.51536/tusbad.1133247 CR - Harvey RA, Lippincott’s IIIustrated Reviews Farmakoloji third eds., Nobel Tıp , İstanbul, Turkey 2009. 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