TY - JOUR T1 - Deciphering the antimicrobial and cytotoxic impact of Aspergillus terreus-biogenic silver nanoparticles AU - Hassane, Abdallah AU - Khalaf, Nourhan AU - Abo-dahab, Nageh AU - El-deeb, Bahig PY - 2025 DA - July Y2 - 2025 DO - 10.12991/jrespharm.1734921 JF - Journal of Research in Pharmacy JO - J. Res. Pharm. PB - Marmara University WT - DergiPark SN - 2630-6344 SP - 1760 EP - 1774 VL - 29 IS - 4 LA - en AB - Nanobiotechnology is a grow-fast applied scientific discipline and has established straight forward shots in medicine, agriculture, and industry. Herein, an investigation on the myco-synthesis of silver nanoparticles (AgNPs) extracellularly by, soil molecularly identified strain, Aspergillus terreus was carried out. Bio-fabricated AgNPs were characterized and inspected for their antibacterial, antifungal, and cytotoxic potency. UV–Visible wave analysis of AgNPs revealed a surface plasmon resonance band at 427 nm. The TEM analysis exhibited spherical particles diameter size ranged between 11 and 30 nm, while AgNPsʼ crystalline nature was confirmed by XRD. Zeta potential value was found to be -16.4 with well dispersed and spherical particles with average size of 27.4 nm. At a concentration of 5000 µg/mL, AgNPs showed antimicrobial efficiency against subjected pathogenic bacterial and Candida species with MICs values ranging from 15.62 to 104.16 µg/mL for antibacterial potency and 125 to 104.16 µg/mL for anticandidal efficacy. Biogenic AgNPs cytotoxicity assay afforded cells viability of 17.8% against HepG2 cell line at 10 µg/mL, meanwhile Artemia salina LC50 mortality was established at 95.32 µg/mL. These findings suggest that biosynthesized AgNPs have promising potent cytotoxicity and antimicrobial issue for treating pathogenic infections. 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