@article{article_1741687, title={Bioactive Secondary Metabolite Profile of Streptomyces netropsis DT02 and Its Biocontrol Potential against Some Phytopathogenic Fungi}, journal={Research in Agricultural Sciences}, pages={1–10}, year={2025}, DOI={10.17097/agricultureatauni.1741687}, author={Yüksel Demirci, Çiğdem and Oskay, Mustafa}, keywords={Biocontrol, LC-HRESIMS, Penicillium italicum, Post-harvest disease, Secondary metabolites, Streptomyces netropsis}, abstract={Actinobacteria represent a significant portion of soil microbial biomass and are renowned for their capacity to produce a wide variety of antibiotics and extracellular enzymes. Recently, researchers have concentrated on Actinobacteria with bioactive secondary metabolites in agriculture as biocontrol agents against phytopathogenic fungi. This study explores the isolation, identification, and metabolic profile of Streptomyces netropsis DT02, and evaluates its antagonistic potential against several phytopathogenic fungi, notably blue mold disease caused by Penicillium italicum in post-harvest citrus fruits. The extract exhibited vigorous antifungal activity in vitro, with inhibition zones ranging from 15 to 45 mm in diameter. The chemical metabolite profile of the crude extract of DT02 was detected using high-performance liquid chromatography-high-resolution electrospray ionization mass spectrometry, and the antifungal compounds were identified based on existing literature. The results showed that DT02 produced 13 different metabolites, including pimprinine, amphotericin B, pimprinethine, N-acetylaureothamine, prothracarcin, and aureothin. The results of these analyses indicated that the antagonistic activity of DT02 is quite substantial. This research creates opportunities for utilising metabolites from DT02 in the biocontrol of some phytopathogenic fungi in crops.}, publisher={Ataturk University}, organization={Manisa Celal Bayar University Scientific Research Projects Coordination Unit}