TY  - JOUR
T1  - Exploring the bioactive potential of peptides derived from the RuBisCO protein in Caulerpa racemosa: an in silico approach
AU  - Windarto, Seto
AU  - Herawati, Vivi Endar
AU  - Wijaya, Yusuf Jati
AU  - Indriati, Dyah Ayu
PY  - 2025
DA  - August
Y2  - 2025
DO  - 10.38042/biotechstudies.1756936
JF  - Biotech Studies
JO  - Biotech Studies
PB  - Tarla Bitkileri Merkez Araştırma Enstitüsü
WT  - DergiPark
SN  - 2687-3761
SP  - 71
EP  - 85
VL  - 34
IS  - 2
LA  - en
AB  - Caulerpa racemosa harbors a rich reservoir of bioactive peptides derived from RuBisCO, a photosynthetic enzyme with promising therapeutic potential. This study aimed to systematically identify and characterize bioactive peptides from C. racemosa RuBisCO using a multi-step in silico pipeline. Simulated proteolysis using 33 enzymes predicted peptides with 35 different biological activities using BIOPEP-UWM. In addition to traditional database screening, further computational filtering was conducted using physicochemical profiling (ExPASy ProtParam), bioactivity prediction (PeptideRanker), toxicity and allergenicity evaluation (ToxinPred, AllergenFP), and structure-based molecular docking against relevant therapeutic targets—angiotensin-I converting enzyme (ACE, PDB: 1O8A) and xanthine oxidase (XO, PDB: 3NRZ). Four peptides with high predicted bioactivity scores (>0.75) showed strong binding affinity (−169.00 to −252.29 kcal/mol) and favorable confidence scores, suggesting their possible use as dual-action therapeutic agents—with both antihypertensive and antioxidant effects. This integrative in silico approach demonstrates the therapeutic relevance of C. racemosa peptides and provides a framework for peptide prioritization prior to experimental validation.
KW  - Bioinformatics
KW  - Computational
KW  - Proteomics
KW  - Seagrapes
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UR  - https://doi.org/10.38042/biotechstudies.1756936
L1  - https://dergipark.org.tr/en/download/article-file/5116831
ER  -