TY - JOUR T1 - Development and validation of a UV-Spectrophotometric method using methanol for the simultaneous estimation of doxycycline and voriconazole in pharmaceutical formulations AU - Bhatia, Dr. Mrs. Neela AU - Ajagekar, Anagha AU - Chougale, Rutuja PY - 2025 DA - September Y2 - 2024 DO - 10.12991/jrespharm.1763367 JF - Journal of Research in Pharmacy JO - J. Res. Pharm. PB - Marmara University WT - DergiPark SN - 2630-6344 SP - 1792 EP - 1801 VL - 29 IS - 5 LA - en AB - The development and validation of a UV-spectrophotometric method for the simultaneous estimation ofdoxycycline and voriconazole in pharmaceutical formulations are presented in this study. This method was designedto address the limitations of existing analytical techniques by providing a simple, accurate, and efficient approachsuitable for routine quality control and therapeutic monitoring. Methanol was selected as the solvent, and estimationwas done using the multiwavelength method of analysis. Estimation of doxycycline was done by recording a differenceof absorbance at 358 and 402 nm while the estimation of voriconazole was done by recording absorbance differenceat 428 and 463 nm. The difference of absorbance for estimation of two drugs is recorded for elimination of interferenceof the other drug. The method demonstrated excellent linearity, with calibration curves showing strong correlationcoefficients. Accuracy was confirmed through satisfactory recovery rates, and precision was validated with %RSDvalues below 2% for both intraday and inter-day measurements. The method exhibited robustness and ruggedness,maintaining consistent performance under varying experimental conditions and across different analysts. Low limitsof detection and quantification underscored the method's sensitivity. This UV-spectrophotometric method offerssignificant practical advantages, including reduced analysis time and cost-effectiveness, making it highly suitable foradoption in pharmaceutical laboratories. The study concludes that this method is a valuable tool for improving drugquality and safety, contributing to advancements in pharmaceutical sciences and supporting enhanced productivity indrug analysis. KW - Simultaneous estimation KW - doxycycline KW - voriconazole KW - method validation KW - UV-spectrophotometry CR - Muller PY, Milton MN. The determination and interpretation of the therapeutic index in drug development. Nat Rev Drug Discov. 2012;11(10):751-761. https://doi.org/10.1038/nrd3801 CR - Mokhtari RB, Homayouni TS, Baluch N, Morgatskaya E, Kumar S, Das B, Yeger H. Combination therapy in combating cancer. Oncotarget. 2017;8(23):38022. https://doi.org/10.18632/oncotarget.16723 CR - Azevedo MM, Teixeira-Santos R, Silva AP, Cruz L, Ricardo E, Pina-Vaz C, Rodrigues AG. 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