@article{article_1787320, title={The amoebicidal effect of New Bioactive o-Phenylenediamine Surface Functionalized Poly (Maleic Anhydride-alt-Vinyl Acetate) and Citric Acid Derived Carbon Quantum Nanodot-Based Conjugates on Acanthamoeba castellanii cysts and trophozoites and its cytotoxic potentials}, journal={Middle Black Sea Journal of Health Science}, volume={11}, pages={383–400}, year={2025}, DOI={10.19127/mbsjohs.1787320}, author={Özpınar, Necati and Karakuş, Nihat and Tunç, Tutku and Khan, Ather Farooq and Karakuş, Gülderen}, keywords={Carbon quantum nanodots (CNDs), maleic anhydride-vinyl acetate alternating copolymer (MAVA), surface modification, o-phenylenediamine (o-PDA), A. castellanii}, abstract={Objective: This study aimed to evaluate the amoebicidal activity of o-phenylenediamine (o-PDA)-conjugated carrier systems, including maleic anhydride-vinyl acetate alternating copolymer (MAVA) and citric acid-derived carbon quantum dots (CNDs), against Acanthamoeba castellanii cysts and trophozoites. Additionally, the cytotoxicity of these compounds was assessed on the HT-22 Mouse Hippocampal Neuronal cell line. Method: Two non-toxic carrier systems were synthesized: MAVA via charge transfer complex (CTC) radical polymerization, and CNDs via rapid thermal pyrolysis of citric acid. o-PDA surface conjugation was achieved through an amide mechanism after the ring-opening reaction. The synthesized compounds were tested for their amoebicidal effects on A. castellanii cysts and trophozoites, while cytotoxicity analyses were conducted using the HT-22 neuronal cell line. Results: According to our findings, the cytotoxicity of all compounds at the tested concentrations was within an acceptable range. When examining the amoebicidal activities, it was observed that MAVA at concentrations of 0.01 g/mL and 0.005 g/mL did not have any viable trophozoites, while CNDs at a concentration of 0.01 g/mL did not have any viable cysts. Overall, all compounds showed significant amoebicidal activity. Conclusion: Our findings demonstrate a strong dose-dependent amoebicidal effect of these substances on A. castellanii cysts and trophozoites. It is believed that these substances, supported by in vivo experiments, could potentially serve as new therapeutic agents.}, number={4}, publisher={Ordu University}, organization={no}