TY - JOUR TT - Bafa Gölü'ndeki Nodularia spumigena Mertens in Jungens (1822)'in morfolojik, moleküler ve toksikolojik karakterizasyonu AU - Tüney Kızılkaya, İnci AU - Demirel, Zeliha AU - Kesici, Kutsal AU - Kesici, Erol AU - Sukatar, Atakan PY - 2016 DA - August JF - Sinop Üniversitesi Fen Bilimleri Dergisi JO - Sinop Uni J Nat Sci PB - Sinop University WT - DergiPark SN - 2536-4383 SP - 39 EP - 52 VL - 1 IS - 1 KW - lake Bafa KW - cyanobacteria KW - HPLC KW - nodularin KW - ELISA N2 - Aşırı alg çoğalmasıoluşturan Nodularia spumigena, hepatotoksikpeptid nodularini üretir. Buçalışmada Haziran ayında Bafa Gölünden izole edilen N. spumigena’nın karakterizasyonuiçin gerçekleştirilen morfolojik, moleküler ve kimyasal analizler uygulanmıştır. Filogenetikanalizler 16S rDNA, fikosiyanin intergenik spacer (IGS) bölgesi ve nodularinsentez gen kümesi (nda) çoğaltılarak gerçekleştirilmiştir.N. spumigena’nın ürettiği nodularinintayini enzyme-linked immunosorbentassay (ELISA), high performance liquid chromatography (HPLC) ve liquidchromatography/mass spectrometry (LC/MS) yöntemleriyle araştırılmıştır.Bu çalışma BafaGölü’nde toksik N. spumigena’nın ilk karakterizasyonudur. CR - [42] Bolch CJS, Orr PT, Jones GJ, Blackburn SI, 1999. Genetic, morphological, and toxicological variation among globally distributed strains of Nodularia (cyanobacteria). J Phycol 35: 339–355. CR - [41] Yabanli M, Turk N, Tenekecioglu E, Uludag R, 2011. A research on massive fish death in Lake Bafa. SAU Fen Bilimleri Dergisi 15: 36–40. CR - [40] Henriksen P, 2005. Estimating nodularin content of cyanobacterial blooms from abundance of Nodularia spumigena and its characteristic pigments- a case study from Baltic entrance area. Harmful Algae 4: 167–178. 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