TY  - JOUR
T1  - Cevizde Yeni SSR Markörlerin Geliştirilmesi
TT  - Development of Simple Sequence Repeat (SSR) Markers from Walnut (Juglans regia L.)
AU  - Topçu, Hayat
AU  - Ikhsan, Adi Surya
AU  - Sütyemez, Mehmet
AU  - Çoban, Nergiz
AU  - Güney, Murat
AU  - Kafkas, Salih
PY  - 2016
DA  - March
Y2  - 2016
JF  - Bahçe
PB  - Atatürk Horticultural Central Research Institute
WT  - DergiPark
SN  - 1300-8943
SP  - 207
EP  - 211
VL  - 45
IS  - (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi
LA  - tr
AB  - Bu çalışmada ceviz için yeni SSR markörlerin geliştirilmesi amaçlanmıştır. Bunun için Maraş-18 ceviz çeşidi DNA’sı kullanılarak oluşturulan genomik kütüphane dört farklı motif ile (CA, GA, AAC ve AAG) zenginleştirilmiştir. Sekansı yapılan 620 klondan 276 adet primer çifti dizayn edilmiştir. Öncelikle gradient PCR ile her bir primer çiftinin DNA’ya bağlanma sıcaklıkları belirlenmiştir. Bant veren 247 SSR primer çifti 15 ceviz çeşidinde analiz edilerek polimorfizm durumları belirlenmiştir. Toplam 185 primer çifti polimorfizm göstermiş olup bunların 80 tanesi (%44.4) GA kütüphanesinden elde edilmiştir. En az polimorfik primer çifti (14 adet) ise AAC kütüphanesinden elde edilmiştir. CA ve AAG kütüphanelerinden ise benzer oranda polimorfizm elde edilmiştir. Sonuç olarak, cevizde bundan sonra yapılacak genetik kaynakların karakterizasyonu, genetik haritalama, popülasyon genetiği gibi çalışmalarda kullanmak üzere çok sayıda yeni SSR markörleri geliştirilmiştir.
KW  - Ceviz
KW  - Juglans regia
KW  - SSR
KW  - mikrosatellit
N2  - In this study, it was aimed to develop SSR markers for walnut. Genomic libraries enriched with CA, GA, AAC and AAG repeats were constructed using genomic DNA of Juglans regia cv. Maraş-18 to develop SSR markers for walnut. A total of 624 clones were sequenced and 276 primer pairs were designed. Amplification of SSR primer pairs was tested by gradient PCR to determine their optimum annealing temperatures, 247 generated PCR products, and 185 polymorphic loci were obtained by characterizing 15 walnut cultivars. A total of 185 primer pairs showed polymorphism and 80 of them obtained from GA-enriched library. The least polymorphism  (14 primer) obtained from AAC-enriched library. CA and AAG enriched libraries produced similar polymorphism rates. As a result, the new SSR primer pairs developed in this study can be used in different genetic studies in walnut such as characterization of genetic resources, construction of genetic linkage maps, and population genetics.
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UR  - https://dergipark.org.tr/en/pub/bahce/issue//1797160
L1  - https://dergipark.org.tr/en/download/article-file/5301603
ER  -