@article{article_1814447, title={Determination of changes in biodiesel production by Gloeocystis vesiculosa cultured in wastewater with the artificial sweetener aspartame and its metabolites}, journal={Aquatic Research}, volume={9}, pages={78–89}, year={2026}, DOI={10.3153/AR26008}, url={https://izlik.org/JA86HY88DN}, author={Onay, Melih}, keywords={Biodiesel, Gloeocystis vesiculosa, Aspartame, Wastewater, Antioxidant activity}, abstract={<p>Microalgae can grow and form biomass in wastewater to produce various end products. In this study, the biomass, lipid content, and lipid yield changes of Gloeocystis vesiculosa cultured in wastewater containing different concentrations of the artificial sweetener APM and its metabolites DKP and PHE were evaluated. The highest biomass amount was 1080 ± 20 mg/L at 250 mg/L of DKP. Conversely, the highest lipid percentage was 31 ± 2% at 250 mg/L APM. There was no significant difference in lipid yield due to the lower biomass content relative to the higher lipid percentages. APM acts as a stress trigger. In addition, the antioxidant enzyme activities of Gloeocystis vesiculosa in wastewater with artificial sweeteners were determined. At 250 mg/L APM, the highest levels of SOD, CAT, APX, and MDA activity were 91 ± 4 U/mg protein, 78 ± 4 U/mg protein, 18 ± 2 U/mg protein, and 3.9 ± 0.2 nmol/mg protein. Future studies using a large-scale photobioreactor will investigate the effects of Gloeocystis vesiculosa on biodiesel production and wastewater treatment in wastewater contaminated with APM, DKP, and PHE. </p>}, number={2}