TY  - JOUR
T1  - Phytochemical profiling and assessment of antioxidant and antibacterial activities of the green macroalga Caulerpa prolifera from the Antalya coast
TT  - Antalya kıyılarından toplanan yeşil makroalg Caulerpa prolifera’nın fitokimyasal profili, antioksidan ve antibakteriyel aktivite değerlendirmesi
AU  - Gümüş, Numan Emre
AU  - Keskinkaya, Hatice Banu
AU  - Okudan, Emine Şükran
AU  - Yılmaz, Bahar
AU  - Koçak, Merve
PY  - 2026
DA  - March
Y2  - 2026
DO  - 10.12714/egejfas.43.1.08
JF  - Ege Journal of Fisheries and Aquatic Sciences
JO  - EgeJFAS
PB  - Ege University
WT  - DergiPark
SN  - 2148-3140
SP  - 68
EP  - 75
VL  - 43
IS  - 1
LA  - en
AB  - Macroalgae are increasingly supported for utilization and development in pharmacology, food, and biotechnology due to their high bioactive potential. This study aimed to characterize the phytochemical profile of C. prolifera extracts and to evaluate their in vitro antioxidant and antibacterial activities. The extracts exhibited total phenolic (TPC) and total flavonoid contents (TFC) values of 2.68±0.22 and 18.94±0.68 µg GAEs/mg, while flavonoid contents were determined as 51.05 and 7.26±0.09 µg QEs/mg. HPLC-DAD analysis identified major metabolites including chlorogenic acid, 4-hydroxybenzoic acid, t-ferulic acid, hydroxycinnamic acid, naringin, o-coumaric acid, rosmarinic acid, salicylic acid, quercetin, t-cinnamic acid, rutin, and naringenin. Antibacterial evaluation demonstrated variable susceptibility among Gram-negative and Gram-positive strains, with MIC values ranging from 7.5 to 5.25 mg/mL. While the ethanol extract exhibited the strongest antioxidant activity in the ABTS•⁺ (IC50: 333.16±0.74 µg/mL) and CUPRAC (A0.50: 538.5±0.02) assays, all extracts showed limited DPPH scavenging capacity (IC50:>800 µg/mL). The ethanolic extract also demonstrated the highest metal chelating capacity (IC50: 327.15±0.63 µg/mL). In conclusion, this study demonstrates that ethanol is the most effective solvent for recovering antioxidant bioactive components from C. prolifera and recommends its use in extraction-based studies.
KW  - Bioactive metabolites
KW  - HPLC-DAD
KW  - phenolic compounds
N2  - Makroalglerin yüksek biyoaktif potansiyelleri, farmakoloji, gıda ve biyoteknoloji alanlarında kullanım ve geliştirilmesini giderek daha fazla desteklemektedir. Bu çalışma, C. prolifera ekstrelerin fitokimyasal profilini karakterize etmeyi ve in vitro antioksidan ve antibakteriyel aktivitelerini değerlendirmeyi amaçlamıştır. Ekstrelerin toplam fenolik içerik (TPC) ve toplam flavonoid içeriği (TFC) değerleri sırasıyla 2.68±0.22 ve 18.94±0.68 µg GAEs/mg olarak, flavonoid içerikleri ise 51.05 ve 7.26±0.09 µg QEs/mg olarak belirlenmiştir. HPLC-DAD analizi, klorojenik asit, 4-hidroksibenzoik asit, t-ferulik asit, hidroksisinamik asit, naringin, o-kumarik asit, rosmarinik asit, salisilik asit, kuersetin, t-sinamik asit, rutin ve naringenin gibi başlıca metabolitleri ortaya koymuştur. Antibakteriyel değerlendirme, Gram-negatif ve Gram-pozitif suşların farklı duyarlılık düzeyleri sergilediğini göstermiştir (MIC: 7.5–5.25 mg/mL). Etanol ekstresi, ABTS•⁺ (IC50: 333.16±0.74 µg/mL) ve CUPRAC (A0.50: 538.5±0.02) testlerinde en yüksek antioksidan aktiviteyi gösterirken, tüm ekstreler DPPH radikal süpürme kapasitesi açısından düşük aktivite sergilemiştir (IC50: >800 µg/mL). En yüksek metal şelatlama aktivitesi de etanol ekstresinde gözlenmiştir (IC50: 327.15±0.63 µg/mL). Sonuç olarak bu çalışmada, etanolün C. prolifera'dan antioksidan biyoaktif bileşenleri geri kazanmak için en etkili çözücü olduğunu göstermektedir.
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UR  - https://doi.org/10.12714/egejfas.43.1.08
L1  - https://dergipark.org.tr/en/download/article-file/5419590
ER  -