TY  - JOUR
T1  - Epigenome Editing: Emerging Tools, Therapeutic Applications, and Challenges in Human Disease Treatment
AU  - Rahimpour, Azam
AU  - Mirahmadi, Maryam
AU  - Hashemi, Nader
AU  - Tabatabaee, Sayed Hassan
AU  - Shams, Forough
AU  - Teng, Yong
PY  - 2025
DA  - November
Y2  - 2025
JF  - Biotech Studies
JO  - Biotech Studies
PB  - Tarla Bitkileri Merkez Araştırma Enstitüsü
WT  - DergiPark
SN  - 2687-3761
SP  - 13
EP  - 34
VL  - 35
IS  - 2
LA  - en
AB  - Epigenetic modifications, including histone alterations, non-coding RNA interactions, and DNA methylation, regulate gene expression without altering the underlying DNA sequence. These modifications are essential for normal biological processes; however, their aberrant regulation is linked to numerous life-threatening disorders. Genome editing nucleases such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas systems offer promising tools for the precise correction of epigenetic abnormalities. This review explores epigenetic mechanisms, genome editing technologies for epigenetic modulation, and their applications in disease contexts, such as cancer and neurodegeneration, with reference to both in vitro and in vivo studies demonstrating therapeutic potential. For instance, aberrant histone acetylation and methylation patterns are frequently observed in cancer. Abnormal DNA methylation and disruptions in histone modifications have been implicated in neurological disorders, such as Alzheimer’s and Huntington’s disease. Although ZFNs and TALENs are foundational tools, their use has been limited by challenges in protein engineering and nonspecific targeting. CRISPR/Cas systems have become a versatile platform. Catalytically inactive Cas9 (dCas9) can be fused to epigenetic editing domains, such as histone deacetylases and DNA methyltransferases, to precisely regulate gene expression. For example, dCas9 has been used to reactivate the BRCA1 tumor suppressor gene in cancer cells. Although epigenetic editing holds significant promise in biomedical research and precision medicine, several challenges remain. These include unintended epigenetic alterations, the efficient delivery of editing tools to target cells, and limited in vivo validation. Future studies using animal models are essential to evaluate the translational potential and clinical applicability of this approach.
KW  - Epigenetic
KW  - Genome editing
KW  - CRISPR/Cas
KW  - TALEN
KW  - ZFN
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UR  - https://dergipark.org.tr/en/pub/biotechstudies/issue//1830114
L1  - https://dergipark.org.tr/en/download/article-file/5450471
ER  -