@article{article_188217, title={Glycyl-L-Histidyl-L-Liysine-Cu(2+) loaded liposome formulations}, journal={Marmara Pharmaceutical Journal}, volume={14}, pages={91–97}, year={2014}, DOI={10.12991/mpj.70317}, author={Erdem, Setenay and Türkoğlu, Murat}, abstract={<p>ABSTRACT: Enhancement of collagen synthesis by glycyl-l-histidyl-l-lysine-Cu2+ (GHK-Cu) <br />derivatives is well known. The different activities of GHK-Cu would be of interest for cosmetic <br />applications. Liposomes provide many benefits as topical drug delivery systems. <br />Structure of double layer and lipid composition of liposomes keep the active substance <br />longer in skin and provide regularly release to the deeper skin layers. Our aim in this study <br />was to prepare GHK-Cu loaded liposomes and characterize them to use in a cosmetic formulation. <br />UV spectrophotometric method was used to detect the GHK-Cu in aqueous medium <br />and FTIR spectrums were taken to determine the absorption bands. In stability studies, it <br />was observed that aqueous solutions of GHK-Cu samples maintained their stability at 4oC <br />for 4 months and the FTIR absorption bands of powdered GHK-Cu did not change when <br />stored under the same stability conditions with aqueous samples. Different liposome formulations <br />were prepared by lipid film hydration technique using different kinds of phospholipids <br />(dipalmitoylphosphatidylcholine (DPPC-5911), Epicuron 100H, and Epicuron 200SH). <br />The particle size and shape of liposomes were determined using microscope, SEM, and laser <br />diffraction method. The average particle size was found to be 13 </p>}, number={2}, publisher={Marmara University}