@article{article_188317, title={Tadalafil attenuates spinal cord injury induced oxidative organ damage in rats}, journal={Marmara Pharmaceutical Journal}, volume={18}, pages={49–55}, year={2014}, DOI={10.12991/mpj.2014186119}, author={Şener, Tarık and Tınay, İlker and Akbal, Cem and Erşahin, Mehmet and Çevik, Özge and Çadırcı, Selin and Çilingir, Özlem and Çetinel, Şule and Şener, Göksel}, keywords={tadalafil; spinal cord injury; anti-inflammatory; spinal cord, kidney; bladder.}, abstract={<p>Spinal cord injury (SCI) has been shown to cause systemic <br />inflammatory response syndrome (SIRS) which damages <br />multiple organs due to an influx of inflammatory cells from the <br />circulation. In this study, we evaluated the effect of tadalafil, a <br />phoshodiesterase inhibitor, against spinal cord, kidney and <br />bladder damage in experimental animal model of spinal cord <br />injury. Male Wistar albino rats were divided into sham-operated <br />control, and either vehicle or tadalafil-treated SCI groups. In <br />order to induce SCI, a standard weight-drop method that <br />induced a moderately severe injury (100 g/cm force) at T10, <br />was used. Injured animals were given either 10 mg/kg tadalafil <br />or vehicle 15 minutes post injury and repeated daily for seven <br />days. After decapitation spinal cord, kidney and bladder tissue <br />samples were obtained to examine oxidative tissue injury; <br />malondialdehyde (MDA) and glutathione (GSH) levels, and <br />superoxide dismutase (SOD), myeloperoxidase (MPO) and <br />caspase-3 activities. Tissues were also examined histologically. <br />In the injured animals, MDA levels MPO and caspase-3 <br />activities in tissues were found significantly increased while <br />tadalafil treatment reversed these increases. On the other hand <br />SCI-induced decreases in GSH levels and SOD activities were <br />also reversed with tadalafil treatment. According to the results, <br />tadalafil exerts beneficial effects against SCI-induced <br />oxidative damage in spinal cord and also in remote organs <br />such as kidney and bladder tissues through its antiinflammatory <br />and antioxidant effects. <br />Key words: tadalafil; spinal cord </p>}, number={2}, publisher={Marmara University}