TY  - JOUR
T1  - Development and Validation of HPLC Method for Determination of Nateglinide in Drug Substances
TT  - ORIGINAL RESEARCH
AU  - Hacıoğlu, Aylin
AU  - Çıtlak, Ayşin
AU  - Karakuş, Sevgi
PY  - 2015
DA  - April
DO  - 10.12991/mpj.2015199640
JF  - Marmara Pharmaceutical Journal
JO  - J Res Pharm
PB  - Marmara University
WT  - DergiPark
SN  - 1309-0801
SP  - 103
EP  - 108
VL  - 19
IS  - 2
LA  - en
AB  - The scope of this study was the development of a reliable, rapid, simple, sensitive, precise reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for determination of assay of the Nateglinide drug substances. The method was developed on ACE C18 analytical column (150mm x 4.6 mm i.d., particle size 5.0 µm) using a mobile phase of acetonitrile and 0.05% trifluoroacetic acid (25:25, v/v). The elute was monitored with the UV detector at 210 nm with a flow rate of 1.5 mL/min. Calibration curve was linear over the concentration range of 0,2846-1,0125 mg/mL. The retention time of Nateglinide was 7.07 min and the correlation coefficient (r) of the regression equation for the Nateglinide was greater than 0.99 in all cases.KEYWORDS :Nateglinide, HPLC, assay, method validation
KW  - Nateglinide
KW  - HPLC assay
KW  - method validation
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UR  - https://doi.org/10.12991/mpj.2015199640
L1  - https://dergipark.org.tr/en/download/article-file/166060
ER  -