TY  - JOUR
T1  - Antioxidant, antimicrobial activities and total flavonoid contents of Cirsium bulgaricum DC. leaf extracts
TT  - ORIGINAL RESEARCH
AU  - Karasakal, Ayça
AU  - Demirci, A.şükrü
AU  - Tokatlı Demirok, Nazan
AU  - Cabı, Evren
PY  - 2015
DA  - January
DO  - 10.12991/mpj.2015198609
JF  - Marmara Pharmaceutical Journal
JO  - J Res Pharm
PB  - Marmara University
WT  - DergiPark
SN  - 1309-0801
SP  - 43
EP  - 51
VL  - 19
IS  - 1
LA  - en
AB  - In this study, the antioxidant activities of methanol andacetone extracts of Cirsium bulgaricum DC. (Asteraceae)were evaluated by five antioxidant assays, includingphosphomolybdate method, cupric ion reducing capacity(CUPRAC), 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonicacid)/persulfate) ABTS/Persulfate, N, N-dimethyl-pphenylenediamine(DMPD) and a Ce(IV)-based reducingcapacity (CERAC) methods. The 80% methanol and acetoneextracts showed 0.005-6.16±0.03 mmol/g and 0.004-5.02±0.02 [(trolox (TR), catechin (CT), epicatechin (EC),rutin (RT), quercetin (QR), naringin (NG) in CUPRAC andABTS/Persulfate methods, respectively]. According to DMPDmethods, inhibition effect were showed 2.3%±0.02 for 80%methanol and 4.6%±0.07 for acetone. Ascorbic acid equivalentmmol (AA)/g extract in CERAC method was found to be0.0834±0.09 for 80% methanol and 0.5622±0.05 for acetone.Ascorbic acid equivalent μg ascorbic acid (AA) /mL extractwere found 807.6±0.07 for 80% methanol and 292.4±0.01 foracetone in phpsphomolybdate method. In the addition to theantioxidant activity of the extract, the total flavonoid contentwas measured. Total flavonoid content of 80% methanol andacetone extracts was found to be 105.5-645±0.04 μg/mlquercetin and rutin equivalent. For the determination ofantibacterial activities Escherichia coli (E.coli) ATCC 25922,Escherichia coli(E.coli) O157:H7 ATCC 33150, SalmonellaE n t e r i t i d i s(S.E n t e r i t i d i s ) AT C C 1 3 0 7 6 , Listeriamonocytogenes(L.monocytogenes) AT C C 7 6 4 4 ,Staphylococcus aureus(S.aureus) ATCC 25923 bacteria wereused as test bacteria. Acetone extracts showed antibacterialactivity against all of the tested bacteria ranged between17.00±1.72- 19.88±1.75 mm and 80% methanol extractsshowed antibacterial activity against all of the tested bacteriaranged between 11.35±1.43-14.43±1.32 mm.Keywords: Antioxidant Activity, Antimicrobial Activity,Total Flavonoid Contents, Cirsium bulgaricum
KW  - Antioxidant Activity
KW  - Antimicrobial Activity
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UR  - https://doi.org/10.12991/mpj.2015198609
L1  - https://dergipark.org.tr/en/download/article-file/166073
ER  -