TY  - JOUR
T1  - In which period of skeletal muscle ischemia-reperfusion injury is local hypothermia more effective?
TT  - İskelet kası iskemi-reperfüzyon hasarının hangi evresinde lokal soğuk uygulaması daha etkilidir?
AU  - Ege, Ahmet
AU  - Turhan, Egemen
AU  - Bektas, Sibel
AU  - Pamuk, Kamuran
AU  - Bayar, Ahmet
AU  - Keser, Selcuk
PY  - 2008
DA  - August
JF  - Acta Orthopaedica et Traumatologica Turcica
PB  - Turkish Association of Orthopaedics and Traumatology
WT  - DergiPark
SN  - 1017-995X
SP  - 193
EP  - 200
VL  - 42
IS  - 3
LA  - en
AB  - Objectives: We investigated the potential beneficial effects of local hypothermia applied during different periods of ischemia-reperfusion injury in a rat model.Methods: An isolated gracilis muscle model of ischemiareperfusion injury was used consisting of four groups, each with six rats. Ischemic injury was induced by clamping the femoral artery for six hours. Local hypothermia at 10 °C was applied during only ischemia (IH), during reperfusion (RH) for four hours, and during both ischemia and reperfusion (IRH). The control group remained untreated. After 24 hours of perfusion, the rats were sacrificed and the gracilis muscles were removed to determine muscle edema (wet-to-dry weight ratio), polymorphonuclear leukocytes (PMNL), and the percentage of necrosis.Results: Compared to the control group (193.7 ±38.9), the PMNL count was significantly lower in the IH, RH, and IRH groups (111.5±36.2, p<0.004; 82±18.6, p<0.002; 54.5±21.8, p<0.002, respectively). The only significant difference in the PMNL count was between the IH and IRH groups (p<0.004). In all the hypothermia groups, the percentage of necrosis was found significantly less than that of the control group (22.5±5.2% vs 11.7±5.2% in IH, p<0.004; 10.8±3.8% in RH, p<0.004; 6.7±2.6% in IRH, p<0.002).Similarly, muscle edema was significantly decreased in the study groups (control, 2.89±0.46 vs 2.01±0.26 in IH, p<0.001; 1.98±0.34 in RH, p<0.001; 1.97±043 in IRH, p<0.001). There were no significant differences between the three hypothermia groups with respect to the percentage of necrosis and muscle edema.Conclusion: Our results show that local hypothermia applied during ischemic and reperfusion periods is significantly effective in reducing ischemia-reperfusion injury.
KW  - Hypothermia
KW  - induced
KW  - ischemia
KW  - muscle
KW  - skeletal/
blood supply
KW  - rats
KW  - reperfusion injury/prevention & control
N2  - Amaç: Sıçan modelinde lokal hipotermi uygulamasının iskemi-reperfüzyon hasarının farklı evrelerindeki etkileri incelendi.Çalışma planı: Grasilis kası iskemi-reperfüzyon modeli için, her birinde altı sıçan olan dört grup oluşturuldu. Femoral arterin altı saat süreyle klemplenmesiyle iskemik hasar oluşturulan kaslara sadece iskemik dönemde (İH), sadece dört saatlik reperfüzyon döneminde (RH) veya iskemi ve reperfüzyon dönemlerinde (İRH) 10 °C’de lokal hipotermi uygulandı. Kontrol grubuna hipotermi uygulanmadı. Yirmi dört saatlik perfüzyon dönemi sonunda yaşamları sonlandırılan deneklerin grasilis kasları çıkarıldı ve örneklerde kas ödemi (yaş/kuru ağırlık oranı), polimorfonükleer lökosit (PMNL) sayımı ve nekroz yüzdesi incelendi.Sonuçlar: Kontrol grubuyla karşılaştırıldığında (193.7 ±38.9), PMNL sayıları İH grubunda (111.5±36.2; p
UR  - https://dergipark.org.tr/en/pub/aott/issue//191061
L1  - https://dergipark.org.tr/en/download/article-file/168670
L1  - https://dergipark.org.tr/en/download/article-file/168671
ER  -