TY - JOUR T1 - Some anatomical and palynological characteristics of Onobrychis paucijuga Bornm. (Fabaceae) TT - Onobrychis paucijuga Bornm. (Fabaceae)’nın bazı anatomik ve palinolojik özellikleri AU - Sezer, Okan PY - 2026 DA - May Y2 - 2026 DO - 10.46309/biodicon.2026.1920583 JF - Biological Diversity and Conservation JO - BioDiCon PB - Ersin YÜCEL WT - DergiPark SN - 1308-5301 SP - 180 EP - 187 VL - 19 IS - 2 LA - en AB - AbstractPurpose: The present study aims to provide some anatomical and palynological characterization of the endemic species Onobrychis paucijuga Bornm. and to assess its taxonomic significance within the genus.Method: Root and stem anatomical structures were examined using light microscopy. Hand sections obtained from plant materials preserved in 70% ethanol were mounted in glycerin–gelatin and analyzed under a Nikon Eclipse 80i microscope. Pollen morphology was investigated using both Wodehouse and Erdtman methods. In addition, scanning electron microscopy (SEM) was employed to examine exine ornamentation and surface ultrastructure.Findings: The root exhibits a well-developed secondary structure with a multilayered periderm, a wide cortex composed of parenchymatous cells, and a distinct vascular cambium separating the secondary phloem and xylem. Sclerenchymatous fibers occur in the cortical region and near the phloem. The stem shows a typical dicotyledonous structure with a collenchymatous hypodermis, multilayered lacunar collenchyma, and well-developed vascular tissues. In older stems, pith parenchyma cells tend to disintegrate. Pollen grains are radially symmetrical, isopolar, and tricolpate. Based on the P/E ratio, they are prolate in the Wodehouse preparation and subprolate in the Erdtman preparation. The exine ornamentation is reticulate-granulate, and the exine is tectate, with a thin tectum. SEM observations confirmed the reticulate-granulate exine ornamentation and revealed detailed surface structures.Conclusion: The anatomical and palynological features of O. paucijuga are largely consistent with those reported for other members of the genus Onobrychis and the family Fabaceae. The combination of anatomical and pollen morphological characters provides valuable diagnostic traits for the identification and taxonomic evaluation of this endemic species. KW - Fabaceae KW - Onobrychis paucijuga KW - anatomy KW - palynology KW - pollen morphology KW - SEM N2 - ÖzetAmaç: Bu çalışma, endemik tür Onobrychis paucijuga Bornm. (Fabaceae)’nın bazı anatomik ve palinolojik özelliklerini ortaya koymayı ve bu özelliklerin cins içerisindeki taksonomik önemini değerlendirmeyi amaçlamaktadır.Metod: Kök ve gövde anatomik yapıları ışık mikroskobu kullanılarak incelenmiştir. %70 etanolde muhafaza edilen bitki materyallerinden elde edilen el kesitleri gliserin-jelatin ile preparat haline getirilmiş ve Nikon Eclipse 80i mikroskobunda analiz edilmiştir. Polen morfolojisi Wodehouse ve Erdtman yöntemleri kullanılarak incelenmiştir. Ayrıca, ekzin ornamentasyonu ve yüzey ultrastrüktürünü belirlemek amacıyla taramalı elektron mikroskobu (SEM) kullanılmıştır.Bulgular: Kök, çok katlı periderm, parenkimatik hücrelerden oluşan geniş bir korteks ve sekonder floem ile ksilemi ayıran belirgin bir vasküler kambiyum ile karakterize edilen iyi gelişmiş bir sekonder yapıya sahiptir. Korteks bölgesinde ve floeme yakın alanlarda sklerenkimatik lifler gözlenmiştir. Gövde, kollenkimatik hipodermis, çok katlı laküner kollenkima ve iyi gelişmiş iletim dokularını içeren tipik bir dikotil yapı göstermektedir. Yaşlı gövdelerde öz parenkima hücrelerinin parçalanma eğiliminde olduğu belirlenmiştir. Polen taneleri radyal simetrik, izopolar ve trikolpattır. P/E oranına göre, Wodehouse preparasyonunda prolat, Erdtman preparasyonunda ise subprolat şekil göstermektedir. Ekzin ornamentasyonu retikülat-granülat olup, ekzin ince bir tektuma sahip tektat yapıdadır. SEM gözlemleri, retikülat-granülat ekzin ornamentasyonunu doğrulamış ve yüzey yapılarına ilişkin ayrıntılı bilgiler ortaya koymuştur.Sonuç: O. paucijuga’nın anatomik ve palinolojik özellikleri, Onobrychis cinsinin diğer üyeleri ve Fabaceae familyası için bildirilen genel özelliklerle büyük ölçüde uyumludur. Anatomik ve polen morfolojik karakterlerin birlikte değerlendirilmesi, bu endemik türün tanımlanması ve taksonomik değerlendirilmesi açısından önemli ayırt edici özellikler sunmaktadır. CR - [1] Legume Phylogeny Working Group. (2017). A new subfamily classification of the Leguminosae based on a taxonomically comprehensive phylogeny. Taxon, 66(1), 44–77. https://doi.org/10.12705/661.3 CR - [2] Graham, P. H., & Vance, C. P. (2003). Legumes: importance and constraints to greater use. Plant physiology, 131(3), 872–877. https://doi.org/10.1104/pp.017004 CR - [3] Sprent, J. I., Ardley, J., & James, E. K. (2017). Biogeography of nodulated legumes and their nitrogen‐fixing symbionts. New Phytologist, 215(1), 40–56. https://doi.org/10.1111/nph.14474 CR - [4] Simpson, M. G. (2019). Plant systematics (3rd ed.). Burlington, MA: Academic Press. CR - [5] Lewis, G., Schrire, B., Mackinder, B., & Lock, M. (Eds.). (2005). Legumes of the world. Kew, UK: Royal Botanic Gardens, Kew. CR - [6] Hedge, I. C. (1970). Onobrychis. In P. H. Davis (Ed.), Flora of Turkey and the East Aegean Islands (Vol. 3, pp. 560–589). Edinburgh, Scotland: Edinburgh University Press. CR - [7] Royal Botanic Gardens, Kew. (2026). Plants of the World Online. Retrieved March 12, 2026, from https://powo.science.kew.org/ CR - [8] Davis, P. H., Mill, R. R., & Tan, K. (Eds.). (1988). Flora of Turkey and the East Aegean Islands (Vol. 10). Edinburgh, Scotland: Edinburgh University Press, pp. 129–131. CR - [9] Yildiz, B., Ciplak, B., & Aktoklu, E. (1999). Fruit morphology of sections of the genus Onobrychis Miller (Fabaceae) and its phylogenetic implications. Israel Journal of Plant Sciences, 47(4), 269–282. https://doi:10.1080/07929978.1999.10676784 CR - [10] Aybeke, M., & Dane, F. (2017). Onobrychis mehmetchiquii (Fabaceae) sp. nov., a new species from European Turkey. Phytotaxa, 298(1), 96–100. https://doi.org/10.11646/phytotaxa.298.1.11 CR - [11] Aytaç, Z., Rabaute, P., & Coulot, P. (2020). Onobrychis silvanensis sp. nov., a new Fabaceae (sect. Hymenobrychis) taxon from Turkey. Phytotaxa, 477(2), 253–260. https://doi.org/10.11646/phytotaxa.477.2.9 CR - [12] Amirabadizadeh, H., Jafari, A., & Mahmoodzadeh, H. (2015). Comparative morphology, anatomy and palynological studies of perennial species of Onobrychis (Fabaceae) in northeast Iran. Nordic Journal of Botany, 33(2), 159–169. https://doi.org/10.1111/njb.00580 CR - [13] Karamian, R., Behjou, A. M., & Ranjbar, M. (2010). Pollen morphology of the Onobrychis sect. Heliobrychis in Iran. Taxonomy and Biosystematics, 1(1), 63–72. CR - [14] Pavlova, D. K., & Manova, V. I. (2000). Pollen morphology of the genera Onobrychis and Hedysarum (Hedysareae, Fabaceae) in Bulgaria. Annales Botanici Fennici, 37(3), 207–217. CR - [15] Talebi, S. M., Azizi, N., Yadegari, P., & Matsyura, A. (2020). Analysis of pollen morphological characteristics in Iranian Onobrychis Miller (Fabaceae) taxa. Brazilian Journal of Botany, 43(3), 609–632. https://doi.org/10.1007/s40415-020-00623-6 CR - [16] Tekin, M., & Yilmaz, G. (2015). Comparative root and stem anatomy of four rare Onobrychis Mill. (Fabaceae) taxa endemic in Turkey. Notulae Scientia Biologicae, 7(3), 308–312. https://doi.org/10.15835/nsb739595 CR - [17] Avcı, S., Sancak, C., Can, A., Acar, A., & Pinar, N. M. (2013). Pollen morphology of the genus Onobrychis (Fabaceae) in Turkey. Turkish Journal of Botany, 37(4), 669–681. https://doi.org/10.3906/bot-1207-52 CR - [18] Taşlıyurt, E., Tuna, M., & Odabaşı, N. Ş. (2023). Pollen morphology of some Onobrychys Mill. (Fabaceae) taxa. Journal of Balkan Science and Technology, 2(2), 48–52. https://doi.org/10.55848/jbst.2023.31 CR - [19] Khan, A., Sultan, A., Shah, S. A., Khan, R., Khan, N., Mumtaz, A. S., ... Khan, T. (2024). Morpho-anatomical and palynotaxonomic study of the genus Onobrychis Miller (Hedysareae—Fabaceae) in Pakistan, and its systematic significance. Phytotaxa, 650(2), 121–147. https://doi.org/10.11646/phytotaxa.650.2.1 CR - [20] Tekin, M., & Yilmaz, G. (2016). Comparative leaf and peduncle anatomy of four Turkish endemic Onobrychis Mill. taxa. Biological Diversity and Conservation, 9(2), 214–220. CR - [21] Esau, K. (1960). Anatomy of seed plants. New York, NY: John Wiley & Sons. CR - [22] Fahn, A. (1982). Plant anatomy (3rd rev. ed.). Oxford, England: Pergamon Press. CR - [23] Faegri, K., & Iversen, J. (1975). Textbook of pollen analysis (3rd rev. ed.). New York, NY: Hafner Press. CR - [24] Wodehouse, R. P. (1935). Pollen grains: Their structure, identification, and significance in science and medicine. New York, NY: McGraw-Hill Book Company. CR - [25] Erdtman, G. (1969). Handbook of palynology: Morphology, taxonomy, ecology: An introduction to the study of pollen grains and spores. New York, NY: Hafner. UR - https://doi.org/10.46309/biodicon.2026.1920583 L1 - https://dergipark.org.tr/en/download/article-file/5866937 ER -