TY - JOUR T1 - Comparison of Chemical Composition, In Vitro Gas Production, and Fermentation Characteristics of Four Different Silages Cultivated in Kahramanmaraş AU - Bakır, Tuğba AU - Selçuk, Bilal AU - Bilal, Yakup PY - 2026 DA - June Y2 - 2026 DO - 10.46897/livestockstudies.1926973 JF - Livestock Studies PB - Uluslararası Hayvancılık Araştırma ve Eğitim Merkezi Müdürlüğü WT - DergiPark SN - 2757-8240 SP - 35 EP - 40 VL - 66 IS - 1 LA - en AB - This study aimed to compare the chemical composition, in vitro gas production, metabolizable energy, and rumen fermentation characteristics of four silage materials (maize, sorghum, maralfalfa, and sugar beet pulp) grown in Kahramanmaraş province. Crude protein, crude fat, crude ash, NDF, and ADF contents were determined using standard analytical methods. In vitro gas and methane production were measured using the Menke technique, and organic matter digestibility, metabolizable energy, true digestible dry matter, and microbial protein production were calculated.Significant differences were observed among silages in terms of chemical composition and fermentation parameters (P < 0.05). Sugar beet pulp silage exhibited the highest gas production (51.63 mL), organic matter digestibility (68.69%), and metabolizable energy (9.84 MJ/kg DM), along with the lowest methane production per unit of fermented feed. Maize silage showed the highest microbial protein production (144.75 mg). Maralfalfa silage was characterized by its high crude protein content (14.08%), whereas sorghum silage had lower digestibility due to its high fiber content.In conclusion, sugar beet pulp silage appears to be a superior energy-rich feed, while maize silage supports efficient rumen microbial activity. These findings highlight the importance of strategic silage selection in optimizing ruminant nutrition. KW - Silage KW - in vitro gas production KW - methane production KW - rumen fermentation KW - metabolizable energy CR - Alhammad, B. A., Mohamed, A., Raza, M. A., Ngie, M., Maitra, S., Seleiman, M. F., & Gitari, H. I. (2023). Optimizing productivity of Buffel and Sudan grasses using optimal nitrogen fertilizer application under arid conditions. Agronomy, 13(8): 2146. https://doi.org/10.3390/agronomy13082146 CR - AOAC. (1990). Official Method of Analysis Association of Official Analytical Chemists (15th ed.). Washington, DC: USA. CR - Blümmel, M., Steingaß, H., & Becker, K. (1997). The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages. British Journal of Nutrition, 77(6): 911– 921. CR - Boğa, M., Kılıç, H. N., Çanga Boğa, D., & Gül Variş, C. (2023). Maralfalfa’nın (Pennisetum Sp.) Ruminant hayvan beslemede kullanımı. İçinde A. Bobat (Ed.), Tarım Orman ve Su BilimLerinde Öncü ve Çağdaş Çalışmalar (ss.191-202). Duvar Yayınları. CR - Choi, H., Sung, J. Y., & Kim, B. G. (2019). Neutral detergent fiber rather than other dietary fiber types as an independent variable increases the accuracy of prediction equation for digestible energy in feeds for growing pigs. Asian-Australasian Journal of Animal Sciences, 33(4), 615. CR - Clark, J. H., Klusmeyer, T. H., & Cameron, M. R. (1992). Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows. Journal of Dairy Science, 75(8), 2304–2323. CR - Duncan, D. B (1955). Multiple range and multiple F tests. Biometrics,11(1): 1-42 CR - García-Rodríguez, J., Ranilla, M.J., France, J., Alaiz-Moretón, H., Carro, M.D., & López, S. (2019). Chemical composition, in vitro digestibility and rumen fermentation kinetics of agro-industrial by-products. Animals, 9(11), 861. https://doi.org/10.3390/ani9110861 CR - Goel, G., Makkar, H.P.S., Becker, K. (2008). Effect of Sesbania sesban and Carduus pycnocephalus leaves and Fenugreek (Trigonella foenum-graecum L.) seeds and their extract on partitioning of nutrients from roughage and concentrate-based feeds to methane. J. Animal Feed Sci. Technol., 147: 72-89. https://doi.org/10.1016/j. anifeedsci.2007.09.010 CR - Huffman, J., Drouin, P., Renaud, J. B., Dunière, L., & LaPointe, G. (2023). Farm management practices and season dependent factors affect the microbial community and chemical profile of corn and grass-legume silages of farms in Ontario, Québec, and Northern New York. Frontiers in Microbiology, 14, 1214915. CR - Jayanegara, A., Wardiman, B., Kondo, M., Ridla, M., Nahrowi, & Laconi, E. B. (2020). Fermentative quality of silage as affected by protein level in the ensiled material: A meta-analysis. In IOP Conference Series: Earth and Environmental Science (Vol. 462, No. 1, p. 012001). IOP Publishing. CR - Johnson, K. A., & Johnson, D. E. (1995). Methane emissions from cattle. Journal of Animal Science, 73(8), 2483– 2492. CR - Khan, N. A., Yu, P., Ali, M., Cone, J. W., & Hendriks, W. H. (2015). Nutritive value of maize silage in relation to dairy cow performance and milk quality. Journal of the Science of Food and Agriculture, 95(2), 238-252. CR - Konca, Y., Beyzi, S. B., Kaliber, M., & Ülger, İ. (2020). Chemical, nutritive, fermentation profile and gas production of citrus pulp silages, alone or combined with maize silage. South African Journal of Animal Science, 50(1), 161-169. CR - Kung, L., & Shaver, R. (2001). Interpretation and use of silage fermentation analysis reports. Focus on Forage, 3(13), 1-5. CR - Lassaletta, L., Estellés, F., Beusen, A. H., Bouwman, L., Calvet, S., Van Grinsven, H. J., Doelman, J. C., Stehfest, E., Uwizeye, A., & Westhoek, H. (2019). Future global pig production systems according to the Shared Socioeconomic Pathways.Science of the Total Environment, 665, 739-751. https://doi.org/10.1016/j.scitotenv.2019.02.079. CR - McDonald, P., Henderson, A. R., & Heron, S. J. E. (1991). The biochemistry of silage, 2nd ed. Chalcombe Publications, Abersytwyth, United Kingdom. CR - Menke, H.H., Steingass, H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28, 7-55. CR - Menke, K.H., Raab, L., Salewski, A., Steingass, H., Fritz, D., Schneider, W., (1979). The estimation of digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they incubated with rumen liquor in vitro. Journal of Agricultural Science (Cambridge) 92, 217-222. https://doi.org/10.1017/ S0021859600086305 CR - Miron, J., Zuckerman, E., Adin, G., Solomon, R., Shoshani, E., Nikbachat, M., & Ben-Ghedalia, D. (2007). Comparison of two forage sorghum varieties with corn and the effect of feeding their silages on eating behavior and lactation performance of dairy cows. Animal Feed Science and Technology, 139(1-2), 23-39. CR - Nursoy, H. (2024). Latest Energy Equations, Nutrient Requirements and Feeding Guidelines Of Cattle. CR - Özdemir, M., & Okumuş, O. (2021). Türkiye'de son beş yılda yapılan bazı silaj çalışmaları. Erciyes Tarım ve Hayvan BilimLeri Dergisi, 4(2), 30-39. CR - Poulsen, M., Jensen, B. B., & Engberg, R. M. (2012). The effect of pectin, corn and wheat starch, inulin and pH on in vitro production of methane, short chain fatty acids and on the microbial community composition in rumen fluid. Anaerobe, 18(1), 83–90. CR - Rankoana, S. A. (2024). Perceptions of climate change impacts on subsistence cattle production. SVU-International Journal of Agricultural Sciences, 6(2), 99-109. CR - Şenyüz, H. H., Şen, G., Ayaş, R., Karapinar, A., & Gümüş, A. E. (2025). Investigation of the nutrient content and IVTD of alfalfa silage with different additives. Turkish Journal of Veterinary Research, 9(2), 131-136. CR - Osorio, F. M. H., Marcelo, A. R. B., Landeo, F. C. T., Zárate, F. T., Montes, M. C., Espilco, P. Q., & Vargas, A. R. R. (2022). Dinámica de crecimiento y valor nutricional del Pennisetum spp (cv. Maralfalfa) en tres edades de corte y dos épocas en el trópico peruano. Revista de Investigaciones Veterinarias del Perú, 33(4). CR - Sun, Z., Li, Y., Li, S., Wang, S., Li, S., Ke, Y., & Yu, Z. (2025). Pretreatment of sweet sorghum silages with Lactobacillus plantarum and cellulase with two different raw material characteristics: Fermentation profile, carbohydrate composition, in vitro rumen fermentation and microbiota communities. Chemical and Biological Technologies in Agriculture, 12(1), 33. CR - Uyarlar, C., Özçınar, Ü., Çetingül, İ. S., Gultepe, E. E., Orman, M. E., Rahman, A., & Bayram, İ. (2025). Comparison of in Situ Digestibility Levels of Pomegranate, Orange, and Sugar Beet Pulp Silage in Buffaloes and Cattle. Kocatepe Veterinary Journal, 18(4), 502-509. CR - Van Soest, P.J., Robertson, J.D. & Lewıs, B.A. (1991). Methods for Dietry Fibre, Neutral Detergent Fibre and NonStarch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74: 3583-3597. CR - Vercoe, P. E., Makkar, H. P. S., & Schlink, A. C. (2010). In vitro screening of plant resources for extra-nutritional attributes in ruminants: Nuclear and related methodologies. Heidelberg: Springer. CR - Zhang, J., Zheng, N., Shen, W., Zhao, S., & Wang, J. (2020). Synchrony degree of dietary energy and nitrogen release influences microbial community, fermentation, and protein synthesis in a rumen simulation system. Microorganisms, 8(2), 231. UR - https://doi.org/10.46897/livestockstudies.1926973 L1 - https://dergipark.org.tr/en/download/article-file/5897105 ER -