TY  - JOUR
T1  - Stereological investigation of the effects of curcumin on the damage in the hippocampus of developing rat brain caused by valproic acid
AU  - Demirel, Burcu
AU  - Eren, Banu
AU  - Sağır, Dilek
PY  - 2014
DA  - September
DO  - 10.5835/jecm.v2i31.5000068733
JF  - Deneysel ve Klinik Tıp Dergisi
JO  - J. Exp. Clin. Med.
PB  - Ondokuz Mayıs University
WT  - DergiPark
SN  - 1309-4483
SP  - 0
VL  - 31
IS  - 2
LA  - en
AB  - Valproic acid is an antiepileptic drug used for the treatment of seizures in newborns,children and adults. Despite having positive effect in the treatment of epilepsy, valproicacid may adversely affect brain function and brain development. Antioxidant and neuroprotectiveproperties of curcumin are well-known. Curcumin has a significant effect inthe treatment of various diseases caused by oxidative stress. In the light of this information,the study aimed to determine the adverse effects of valproic acid in the number ofpyramidal cells in the hippocampus of the developing rat brain and whether curcuminhas protective effects against these adverse effects. A total of 50 Wistar male rats weredivided into two groups as 7 and 14 day old. Each group was subdivided into five groupsas control, curcumin, dimethyl sulfoxide, valproic acid, valproic acid+curcumin. Afterdissolving in 10% dimethylsulfoxide, the rats in each group received intraperitoneal injectionof valproic acid (400 mg/kg) and curcumin (200 mg/kg). Rats were sacrificed24 hours after the last injection. After histological processes, stereological analysis wasperformed using optical fractionation technique. Overall number of neurons in the lefthippocampus (CA1, CA2, CA3 and CA4) of the 7 and 14 day old rats was estimated andaverage numerical values were determined. The mean total neuron numbers and standarddeviations for 7 day old rats were 727224±4531.7 in control group; 312232.8±15853.3 invalproic acid group and 431665.4±28464.5 in valproic acid+curcumin group. These numericalvalues were 508160.6±21985.2; 313958.9±18199.66 and 456028.8±24942.9 for14 day old rats respectively. The results support the idea that curcumin can be a potentialprotective and effective agent in the prevention of histological neuronal damage that mayoccur in the developing rat brain due to valproic acid treatment. We are in the opinion thatthe results of this study will shed light on clinical studies and form a basis to antioxidantstudies involving the use of valproric acid.
KW  - Curcumin; hippocampus; stereology; valproic Acid
UR  - https://doi.org/10.5835/jecm.v2i31.5000068733
L1  - https://dergipark.org.tr/en/download/article-file/190400
ER  -