TY  - JOUR
T1  - Fatty acid-binding protein 2 Ala54Thr polymorphism among psoriatic patients in Çanakkale, Turkey
AU  - Hız, Meliha Merve
AU  - Akı, Cüneyt
AU  - Öğretmen, Zerrin
PY  - 2014
DA  - September
DO  - 10.5835/jecm.v2i31.5000068738
JF  - Deneysel ve Klinik Tıp Dergisi
JO  - J. Exp. Clin. Med.
PB  - Ondokuz Mayıs University
WT  - DergiPark
SN  - 1309-4483
SP  - 0
VL  - 31
IS  - 2
LA  - en
AB  - A single nucleotide polymorphism (G-to-A) in codon 54 of exon two resulting in an alanineto threonine substitution and we hypothesized that, in psoriatic patients Ala54Thrcarriers would have increased risk for psoriasis than Ala54-homozygous counterparts.141 psoriatic patients and 131 healthy individuals who gave informed consent, were enrolledto study. FABP2 polymorphism was evaluated with melting curve analysis (RealTimePCR). Genotype frequencies were estimated by gene counting. In consistencyof genotype frequencies with the Hardy-Weinberg equilibrium was tested using a Chi-Square. Odds ratio (OR) and 95% confidence interval were calculated to estimate therisks related to (FABP2) Ala54Thr polymorphism. To calculate the significance of OR,Fisher’s exact test was used. Eighteen patients (12.8%) had Ala54/Ala54 (wild group)and 118 (83.7%) patients a mutant genotype, Ala54/Thr54 (55 patients, 39%) or Thr54/Thr54 (63 patients, 44.7%). In control group, ten subjects (7.6%) had the genotype Ala54/Ala54 (wild-type group) and 121 (92.4%) participants had the genotype Ala54/Thr54(n=47, 35.9%) or Thr54/Thr54 (n=74, 56.5%). The genotype distribution were consistentwith Hardy-Weinberg equilibrium for psoriatic and healthy groups (χ²calculated: 0.07 2nddegree).When psoriatic patients with homozygote “AA” or heterozygote genotype “GA”were compared with the ones with normal genotype, no statistical correlation was found(ORAAvs GG:0.47; 95%CI: 0.20-1.09; p:0.08 and ORGA vs GG:0.65; 95%CI: 0.27-1.55; p:0.33). Asidethe transition of G to A at codon 54 of FABP2 were not increasedpsoriasis risk in statistically significant manner (OR: 0.68;95% CI: 0.47-0.99; p: 0.046).The overall odds ratio was found 0.697 (p=0.055) by Armitage’s Trend Test. We foundno correlation between allelic distribution or genotype frequency of FABP2 Ala54Thrpolymorphism and psoriasis. In conclusion, our present results suggest that FABP2 genotypeswere not statistically significant and unsuitable to use as dependent determinants ofthe risk. The alanine to threonine substitution of FABP2 gene can’t be used as diagnosticmarker for psoriasis and validation of these findings require to replicate within largescalestudy group to find real association.
KW  - Ala54Thr; FABP2; Psoriasis; rs1799883
UR  - https://doi.org/10.5835/jecm.v2i31.5000068738
L1  - https://dergipark.org.tr/en/download/article-file/190404
ER  -