TY  - JOUR
T1  - Effects of leptin on histomorphometry of liver in high-fat diet fed obese rats
AU  - Kıvrak, Elfide Gizem
AU  - Aydın, İşınsu
AU  - Tümentemur, Gamze
AU  - Altunkaynak, Berrin Zuhal
AU  - Kaplan, Süleyman
PY  - 2014
DA  - September
DO  - 10.5835/jecm.v2i31.5000068748
JF  - Deneysel ve Klinik Tıp Dergisi
JO  - J. Exp. Clin. Med.
PB  - Ondokuz Mayıs University
WT  - DergiPark
SN  - 1309-4483
SP  - 0
VL  - 31
IS  - 2
LA  - en
AB  - Obesity, is one of the most significant and common diseases of today’s world. Leptin,one of the most important adipose-derived hormones and among the best-known hormonemarkers for obesity and it plays a key role in regulating stable body weight andis synthesized and released by the fat cells in adipose tissue. So leptin has used for obesitytreatment. The aim of this study; to detect effects of high fat diet induced obesityand leptin on liver by using physical disector and Cavalieri methods. This study wasincluding four groups: Non-obese control, obese control, non-obese Leptin and obese-Leptin groups. Liver of all animals were prepared and stained with hematoxylin eosinfor stereological and light microscopical analyses. The volume of liver was calculatedby Cavalieri methods and the number of hepatocyte was analyzed by physical dissector.Liver volumes of control and obese groups were significantly different from the eachother. The mean numerical density of hepatocytes for the obese group was significantlydecreased in comparison to the control group. But Leptin application lead to a significantincrease of hepatocyte number in obese-leptin group after fatty diet induced obesity. Innonobese-control and nonobese-leptin group, livers had healthy histological structure.But livers of obese-control group, steatosis, ballooning degeneration and hypertrophiedcells were found. Also between hepatocyte plates, possible dead hepatocytes with smalldark cytoplasm and pycnotic nuclei were seen. In sinusoids, fibrin deposits were detected.In obese-leptin group, steatosis was decreased and any hypertrophic cell or possibledead cells were not observed. Moreover sinusoids didn’t include any deposits. Theseobtained data demonstrated that leptin has a protective effect on the liver in terms ofmorphometrical parameters which are liver volumes and number of hepatocytes and histologicalstructure in fatty diet induced obese rat model.
KW  - High Fat Diet; leptin; mon-alcoholic fatty liver disease; obesity; rat
UR  - https://doi.org/10.5835/jecm.v2i31.5000068748
L1  - https://dergipark.org.tr/en/download/article-file/190414
ER  -