TY  - JOUR
T1  - Effects of diclofenac sodium on rat hippocampus exposed to acute unilateral subdural hematoma: A histological, stereological and molecular study
AU  - Turkmen, Aysın Pınar
AU  - Kaplan, Süleyman
AU  - Aksoy, Abdurahman
AU  - Altunkaynak, Berrin Zuhal
AU  - Yurt, Kübra
AU  - Elibol, Ebru
AU  - Çokluk, Cengiz
AU  - Önger, Mehmet Emin
PY  - 2014
DA  - September
DO  - 10.5835/jecm.v2i31.5000068750
JF  - Deneysel ve Klinik Tıp Dergisi
JO  - J. Exp. Clin. Med.
PB  - Ondokuz Mayıs University
WT  - DergiPark
SN  - 1309-4483
SP  - 0
VL  - 31
IS  - 2
LA  - en
AB  - In this study, it was aimed to evaluate the potential effects of acute subdural hematoma(ASDH) and diclofenac sodium therapy following ASDH on the rat hippocampus by histological,stereological and biochemical methods. For this aim, 24 male Sprague-dawleyrats were used. The subjects were divided into 4 groups. One ml venous blood was collectedfrom the tail vein of the all animals before seven day from experimental acute subduralhematoma. Plasma serotonin, adrenaline and noradrenaline levels were measured inthese plasma samples. During experimental acute subdural hematoma: Non-heparinized0.1 ml of autologous blood from the tail vein of the animals in Group 3 and 4 were givento subdural space. Group 4 daily received intra-muscular diclofenac sodium administrationat 15-mg/kg-dose from the postoperative second hour to 7th day of the operation.Group 1 and 2 used for control and sham operation, respectively. At postoperative 8th day,1 ml venous blood from the all animals was collected before perfusion. The brain of animalswere analysed by histological, stereological and biochemical techniques. The meannumber of neurons in the CA1, CA2, CA3 regions of the hippocampus was estimated.Total number of neurons in the hippocampus of the diclofenac sodium treated group wassignificantly decreased. When comparing of postoperative blood samples, there was nodifference between the levels of adrenaline and serotonin levels among the groups. But,only noradrenalin levels in the treated group it found to be higher than those of the shamand control groups (p<0.05). In this study, it was shown that using of diclofenac sodiumafter acute subdural hematoma caused to loss of the neurons in the hippocampal CA1,CA2, and CA3 regions.
KW  - Acute subdural hematom
KW  - biochemistry; diclofenac sodium; hippocampus; stereology
UR  - https://doi.org/10.5835/jecm.v2i31.5000068750
L1  - https://dergipark.org.tr/en/download/article-file/190416
ER  -