@article{article_237514, title={Evaluation of the geneXpert MTB/RIF assay for early diagnosis of tuberculosis and detection of rifampicin resistance in pulmonary and extrapulmonary specimens}, journal={Cukurova Medical Journal}, volume={41}, pages={548–553}, year={2016}, DOI={10.17826/cukmedj.237514}, author={Albay, Ali and Güney, Mustafa and Tekin, Kemal and Kısa, Özgül and Sığ, Ali Korhan}, keywords={Tuberculosis,GeneXpert MTB/RIF,MDR-TB}, abstract={<p> <span style="color:rgb(98,98,98);font-family:verdana, tahoma, helvetica;font-size:12px;line-height:19px;text-align:justify;">Purpose: The emerging of multi-drug resistant strains of Mycobacterium tuberculosis complex and the resurgence of tuberculosis have forced new methods for diagnosis and antimicrobial susceptibility to be developed. In this study, we evaluated a recently marketed diagnostic technique, GeneXpert MTB/RIF test, in terms of identifying Mycobacterium tuberculosis complex and detecting rifampicin resistance from clinical samples.  </span> <br style="color:rgb(98,98,98);font-family:verdana, tahoma, helvetica;font-size:12px;line-height:19px;text-align:justify;" /> <span style="color:rgb(98,98,98);font-family:verdana, tahoma, helvetica;font-size:12px;line-height:19px;text-align:justify;">Material and Methods: In our study, GeneXpert MTB/RIF assay was compared with conventional and automated culture methods. 389 various clinical samples (171 pulmonary and 218 ekstrapulmonary samples) were cultured with BACTEC MGIT 960 culture system and Löwenstein-Jensen medium and were tested with GeneXpert MTB / RIF test.  </span> <br style="color:rgb(98,98,98);font-family:verdana, tahoma, helvetica;font-size:12px;line-height:19px;text-align:justify;" /> <span style="color:rgb(98,98,98);font-family:verdana, tahoma, helvetica;font-size:12px;line-height:19px;text-align:justify;">Results: 23 samples were positive for Mycobacterium tuberculosis complex by GeneXpert MTB/RIF test, but 22 samples were positive for Löwenstein-Jensen medium and BACTEC MGIT 960 culture system. 2 of 23 samples were detected rifampicin resistant at the first study, but re-studying of these samples resulted as rifampicin resistant for only one sample. According to culture findings of all pulmonary samples, the sensitivity and specificity for GeneXpert MTB / RIF test were both 100% (16/16). The sensitivity and specificity for extrapulmonary samples were 83.3% and 99.5%, respectively. </span> <br style="color:rgb(98,98,98);font-family:verdana, tahoma, helvetica;font-size:12px;line-height:19px;text-align:justify;" /> <span style="color:rgb(98,98,98);font-family:verdana, tahoma, helvetica;font-size:12px;line-height:19px;text-align:justify;">Conclusion: GeneXpert MTB / RIF test is an effectual automated molecular diagnostic technique with its successful and reliable performance in early diagnosis of tuberculosis and detecting multi-drug resistant strains. </span> <br /> </p>}, number={3}, publisher={Cukurova University}