@article{article_237822, title={Determination of P-Glycoprotein Expression by Flow Cytometry in Hematological Malignancies}, journal={Dicle Medical Journal}, volume={43}, pages={33–38}, year={2016}, DOI={10.5798/diclemedj.0921.2016.01.0634}, author={Saraymen, Berkay and Çimen, Behzat and Çetin, İhsan and Köker, Mustafa and Çetin, Aysun and Eser, Bülent}, keywords={Çoklu ilaç direnci-1 geni, AML, ALL, P-glikoprotein, akım sitometri}, abstract={<p>Objective: Determination the expression of P-glycoprotein is especially problematic for normal tissues because immunological methods are limited in terms of sensitivity. We aimed to determine the expression of P-glycoprotein and CD34 by flow cytometry, and to evaluate the level of expression of P-glycoprotein and CD34 with unresponsive to treatment in patients diagnosed with hematologic malignancy. <br />Methods: Our study included fifty patients diagnosed with acute myeloblastic leukemia and acute lymphoblastic leukemia, and twenty healthy controls who were admitted to Erciyes University Hematology-Oncology Hospital. The suspended cells from bone marrow samples of patients and the peripheral blood samples of healthy people were marked with P-glycoprotein phycoerythrin and CD34 FITC or PerCP Cy 5.5; and then surface expression was measured by means of flow cytometry. <br />Results: In 6 of 30 acute myeloblastic leukemia patients P-glycoprotein and CD34 expression, in 6 of 20 acute lymphoblastic leukemia patients P-glycoprotein, in 5 of them CD34 expression were determined. A significant relation between P-glycoprotein and CD34 expressions in acute myeloblastic leukemia and acute lymphoblastic leukemia bone marrow samples was reported. <br />Conclusion: Our data indicate that flow cytometry is more reliable, precise and faster than molecular methods for measuring P-glycoprotein expression and suggests the possibility of a significant relationship between P-glycoprotein and CD34 expressions in acute myeloblastic leukemia and acute lymphoblastic leukemia bone marrow samples. The blast cells expressing CD34 on their surface along with P-glycoprotein simultaneously show that multi drug resistance 1 gene is mostly active in immature cells. <br />Key words: MDR-1 gene, AML, ALL, P-glycoprotein, flow cytometry </p>}, number={1}, publisher={Dicle University}