@article{article_247673, title={Determination of Genotoxic Effects of Organic Insecticide Fipronil in Chick in vivo Micronucleus Assay}, journal={Selcuk University Journal of Science Faculty}, volume={2}, pages={1–8}, year={2011}, author={Özparlak, Haluk and Arslan, Atillla and Güler, Gökhan Özmen}, keywords={Fipronil, chick, genotoxicity, micronucleus, nuclear abnormality}, abstract={Fipronil, an organic insecticide, is a member of relatively new and small class of pesticides, the phenyl pyrazoles or fiproles group of chemicals. The aim of this study was to determine the possible acute and chronic genotoxic effects of commercial formulations of Fipronil in micronucleus test in peripheral blood erythrocytes of the chick. For this purpose, different doses of Fipronil (6.25, 25, 50 and 100 mg/kg) were given to chicks intraperitoneally. Distilled water and cyclophosphamide were also used as negative and positive controls, respectively. After treatments, blood smears were prepared and stained with modified May Grünwald–Giemsa. In this smears, frequencies of micronucleus and nuclear abnormality were examined in erythrocytes under light microscope. A statistically significant increase in the frequency of micronucleus and nuclear abnormality of groups treated with Fipronil could not be observed compared to negative control (p>0.05). These results indicate that commercial formulations of Fipronil at doses tested may be considered as a non–genotoxic compound for chicks.}, number={37}, publisher={Selcuk University}