TY  - JOUR
T1  - Effects of Thidiazuron and Activated Charcoal on in vitro Shoot Proliferation and Rooting of Myrtle (Myrtus communis L.)
TT  - Effects of Thidiazuron and Activated Charcoal on in vitro Shoot Proliferation and Rooting of Myrtle (Myrtus communis L.)
AU  - Şan, Bekir
AU  - Karakurt, Yaşar
AU  - Dönmez, Fatma
PY  - 2015
DA  - March
DO  - 10.15832/tbd.70660
JF  - Journal of Agricultural Sciences
JO  - J Agr Sci-Tarim Bili
PB  - Ankara University
WT  - DergiPark
SN  - 1300-7580
SP  - 177
EP  - 183
VL  - 21
IS  - 2
LA  - en
AB  - In this study, a micropropagation approach was developed for a commercially important myrtle clone ‘Aşı Mersin’ in Turkey. The effects of different concentrations of thidiazuron (TDZ), 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA) on shoot formation and the effect of activated charcoal on rooting were studied. The most of the shoots were obtained on Murashige and Skoog (MS) medium containing 0.3 mg L-1 TDZ + 0.1 mg L-1 NAA in the 1st subculture (3.8 shoot), and from MS with 0.3 mg L-1 TDZ + 0.01 mg L-1 NAA in the 2nd subculture (4.0 shoot). The explants were rooted in ½MS containing indole-3-butyric acid (IBA), NAA and activated charcoal (AC). IBA applications induced more rooting than NAA. The medium supplemented with 1.0 mg L-1 IBA + 2.0 g L-1 AC resulted in the highest rooting ratio (80%). Addition of AC into the medium resulted in slight increase in the rooting ratio, significant increase in shoot length, and reduced darkening in the rooting area. Acclimatization was successful for 86% of the rooted plants
KW  - Myrtle
KW  - Proliferation
KW  - Activated charcoal
KW  - Thidiazuron
KW  - Acclimatization
N2  - Çalışmada, Mersin bitkisinin ticari öneme sahip “Aşı Mersin” klonu için bir mikro çoğaltım yöntemi geliştirilmiştir. Bu amaçla farklı konsantrasyonlarda thidiazuron (TDZ), 6-benzil amino pürin (BAP) ve naftalin asetik asit’in (NAA) sürgün proliferasyonu ve aktif karbonun (AK) köklenme üzerine etkileri belirlenmiştir. En yüksek sürgün sayıları 1. alt kültürde 3.8 adet ile 0.3 mg L-1 TDZ + 0.1 mg L-1 NAA içeren Murashige ve Skoog (MS) ortamından elde edilirken, 2. alt kültürde 4.0 adet ile 0.3 mg L-1 TDZ + 0.01 mg L-1 NAA içeren MS ortamından elde edilmiştir. İn vitro sürgünler indole-3-butyric acid (IBA), NAA ve aktif kömür içeren ½MS ortamında köklendirilmiştir. IBA uygulamalarının NAAuygulamalarına göre köklenmeyi daha fazla uyardığı belirlenmiştir. İçinde 1.0 mg L-1 IBA + 2.0 g L-1 AC bulunan½MS ortamı en yüksek köklenme oranını (% 80) vermiştir. Ortama AC eklenmesi köklenme oranını nispeten ve sürgünuzunluğunu önemli derecede arttırmış ve kök bölgesindeki kararmayı ise azaltmıştır. Köklenmiş bitkilerin % 86’sı dışkoşullara başarılı bir şekilde alıştırılmıştır
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UR  - https://doi.org/10.15832/tbd.70660
L1  - https://dergipark.org.tr/en/download/article-file/21101
ER  -