TY  - JOUR
TT  - Identification and characterization of Turnip mosaic virus (TuMV) infection on  canola plants (Brassica napus L.) in  Çanakkale province
AU  - Karanfil, Ali
AU  - Korkmaz, Savaş
PY  - 2016
DA  - June
JF  - Plant Protection Bulletin
PB  - TARIM VE ORMAN BAKANLIĞI EĞİTİM VE YAYIN DAİRESİ BAŞKANLIĞI
WT  - DergiPark
SN  - 0406-3597
SP  - 0
EP  - 0
VL  - 56
IS  - 2
KW  - Şalgam mozaik virüsü
KW  - kanola; DAS-ELISA; RT-PCR; filogenetik analiz
N2  - This study was carried out in canola plants in Çanakkale province in  2014. For this aim, canola plants showing severe mosaic symptoms were collected  from 2 districts (Ezine and Lapseki) of Çanakkale province in Turkey. Collected  twenty-one samples were tested by DAS-ELISA with polyclonal antisera (Bioreba,  Switzerland) and RT-PCR using gene specific primers containing partial NIb+CP  to determine the presence of Turnip  mosaic virus (TuMV). DAS-ELISA and RT-PCR test results indicated that 15  sample were infected with TuMV. Corresponding 1178 bp DNA fragment of isolate  CK01 chosen randomly was purified and sequenced. The nucleotide sequence of the  partial NIb+CP gene of CK01 isolate was compared with TuMV isolates from  different parts of the world found in gen bank. Sequence analysis of partial  NIb+CP genes showed 88-93% identities among CK01 and world TuMV isolates at  nucleotide level. Phylogenetic relationship was determined among CK01 and TuMV  isolates from different parts of the world. To our knowledge; although TuMV  infections were identified in different plants, this is the first report of  TuMV infection on canola plants in Turkey.
UR  - https://dergipark.org.tr/en/pub/bitkorb/issue//258053
L1  - https://dergipark.org.tr/en/download/article-file/224431
ER  -