TY  - JOUR
TT  - The Effects to GM-CSF Expression and Fibroblast Proliferation of pGMCSF Containing Chitosan/PVP Hydrogels
AU  - Şalva, Emine
AU  - Akbuğa, Jülide
PY  - 2017
DA  - May
DO  - 10.12991/marupj.278854
JF  - Marmara Pharmaceutical Journal
JO  - J Res Pharm
PB  - Marmara University
WT  - DergiPark
SN  - 1309-0801
SP  - 228
EP  - 234
VL  - 21
IS  - 2
KW  - pGM-CSF
KW  - PVP
KW  - kitozan
KW  - hidrojel
N2  - The aim of this study was to develop pGM-CSF containingchitosan/PVP hydrogel system for gene delivery. The hydrogelsin semi-interpenetrating polymer network (semi-IPN)structure were synthesized by glutaraldehyde crosslinkingbetween 2% chitosan and 2%, %4, %6 PVP. The viscosity,swelling properties and surface morphology of hydrogels wereexamined. These hydrogels showed highly porous structure.The porosity was increased with enhanced PVP concentration.Viscosity decreased with increasing PVP concentration of thehydrogels. The swelling percentage increased with increasingPVP concentration. In order to examine the effect of cellattachment and proliferation of hydrogels, NIH-3T3 fibroblastcell was used. The cells were able to attach on the top surfaceof hydrogels. However, the attachment of cells were slow atdays 1 and 3. At days 7 and 14, cells looked healthy and normaland proliferated around and top of the hydrogels. With theELISA study, the highest GM-CSF expression was obtainedwith pGM-CSF containing 2% chitosan+4% PVP hydrogels.MTT assay showed that chitosan/PVP hydrogels did notinduce significant cytotoxic effect. As a result, these hydrogelscould be served candidate for local gene delivery in tissueregeneration.
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UR  - https://doi.org/10.12991/marupj.278854
L1  - https://dergipark.org.tr/en/download/article-file/290663
ER  -