TY - JOUR T1 - Biodiversity of Bacteria Isolated from Different Soils TT - Biodiversity of Bacteria Isolated from Different Soils AU - Yaman, Fatma AU - Aktaş, Betül AU - Touray, Mustapha AU - Poyrazoğlu Çoban, Esin AU - Bıyık, Hacı PY - 2017 DA - January DO - 10.21448/ijsm.278866 JF - International Journal of Secondary Metabolite JO - Int. J. Sec. Metabolite PB - İzzet KARA WT - DergiPark SN - 2148-6905 SP - 27 EP - 34 VL - 4 IS - 1 LA - en AB - The aim of this study was to determine the biodiversity of PHB producingbacteria isolated from soils where fruit and vegetable are cultivated (onion,grape, olive, mulberry and plum) in Aydın providence. Morphological, cultural,biochemical, and molecular methods were used for bacteria identification. Theseisolated bacteria were identified by 16S rRNA sequencing and using BLAST. Thefollowing bacteria Bacillus thuringiensis(6), Bacillus cereus (8), Bacillus anthrachis (1), Bacillus circulans (1), Bacillus weihenstephanensis (1), Pseudomonas putida (1), Azotobacter chroococcum (1), Brevibacterium frigoritolerans (1), Burkholderia sp. (1), Staphylococcus epidermidis (1), Streptomyces exfoliatus (1), Variovorax paradoxus (1) were found. TheMaximum Likelihood method was used to produce a molecular phylogenetic analysisand a phylogenetic tree was constructed. These bacteria can producepolyhydroxybutyrate (PHB) which is an organic polymer with commercial potentialas a biodegradable thermoplastic. PHB can be used instead of petrol derivatednon-degradable plastics. For this reason, PHB producing microorganisms aresubstantial in industry. KW - PHB KW - bacteria KW - 16S rRNA KW - biodiversity KW - soil N2 - The aim of this study was to determine the biodiversity of PHB producing bacteria isolated from soils where fruit and vegetable are cultivated (onion, grape, olive, mulberry and plum) in Aydın providence. Morphological, cultural, biochemical, and molecular methods were used for bacteria identification. These isolated bacteria were identified by 16S rRNA sequencing and using BLAST. The following bacteria Bacillus thuringiensis (6), Bacillus cereus (8), Bacillus anthrachis (1), Bacillus circulans (1), Bacillus weihenstephanensis (1), Pseudomonas putida (1), Azotobacter chroococcum (1), Brevibacterium frigoritolerans (1), Burkholderia sp. (1), Staphylococcus epidermidis (1), Streptomyces exfoliatus (1), Variovorax paradoxus (1) were found. The Maximum Likelihood method was used to produce a molecular phylogenetic analysis and a phylogenetic tree was constructed. 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Sci., 6 (4): 46-51. UR - https://doi.org/10.21448/ijsm.278866 L1 - https://dergipark.org.tr/en/download/article-file/260236 ER -