@article{article_280740, title={Investigation of Vancomycin-Resistant Enterococci and The Distribution of The Vancomycin Resistance Associated Genes in Chickens}, journal={Journal of Anatolian Environmental and Animal Sciences}, volume={1}, pages={92–95}, year={2016}, DOI={10.35229/jaes.280740}, author={Çepoğlu, Haluk and Adıgüzel, Mehmet Cemal and Bağcıgil, Arzu Funda and Ak, Seyyal}, keywords={Chicken,Enterococcus spp.,Faeces,PCR}, abstract={<p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;text-indent:35.4pt;line-height:normal;"> <span lang="en-us" style="font-size:12pt;font-family:’Times New Roman’, serif;" xml:lang="en-us">In this study, chicken litter and cloacal swabs (n=300) were
investigated for the detection of vancomycin-resistant enterococci and determination
of resistance associated genes. The samples were cultured in Enterococcocel
broth and on Enterococcocel agar. One hundred seven presumptive isolates were examined
by PCR at the genus level. For the determination of vancomycin resistance of <i>Enterococcus </i> spp. isolates (n=98), disc
diffusion and macro dilution methods were performed. According to disc
diffusion test, 49 isolates were resistant and 48 were intermediate and according
to the macro-dilution tests, 5 of the isolates were found to be resistant
(MIC≥64 μg/ml), 83 of them were intermediate (MIC = 8-16 μg/ml) and 9 of them susceptible
(MIC≤4 μg/ml). Vancomycin resistant and intermediate isolates were examined for
detection of MIC value for teicoplan (n=88); 2 were resistant (MIC≥32 μg/ml)
and 86 susceptible (MIC≤8 μg/ml). Finally, vancomycin resistant and
intermediate isolates were examined by PCR for the detection of vancomycin
resistance associated genes and identification at species level by PCR. As a
result of PCR, 5 of the isolates were <i>vanA </i>
gene carrying <i>E. faecium </i>, 29 were <i>E. gallinarum </i>with <i>vanC1 </i> gene and 54 were <i>E. casseliflavus </i>with vanC2 <i>/3 </i> gene.  </span> </p> <p> </p>}, number={3}, publisher={Bülent VEREP}