@article{article_283920, title={Effects of Salt Stress and Inoculation Ratios in Cell Cultures of Rubia tinctorum L.}, journal={Sakarya University Journal of Science}, volume={21}, pages={328–334}, year={2017}, DOI={10.16984/saufenbilder.283920}, author={Nartop, Pınar and Akay, Şeref and Gürel, Aynur}, keywords={alizarin,callus,cell suspension,purpurin,Rubia tinctorum L.,salt stress}, abstract={<p class="MsoNormal" style="margin-top:11pt;margin-right:0cm;margin-bottom:11pt;margin-left:0cm;text-align:justify;line-height:150%;"> <span lang="en-us" style="font-size:10pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us">In this study, salt stress was implemented to callus
and cell suspension cultures of <i>Rubia
tinctorum </i> L. at 100 mM and 200 mM concentrations and the effects on fresh
and dry weights (g), relative dry weight ratios, and alizarin and purpurin
contents (mg/g dry weight) of biomass were determined. Due to an increase in
salt concentrations, biomass obtained from callus and cell suspension cultures
were decreased. In callus cultures, due to the low viability and proliferation
rate of the callus cells, the biomass showed no significant increase. Alizarin
and purpurin contents decreased as the salt concentrations in callus cultures
increased, but amounts of these compounds increased in cell suspension
cultures. Inoculation </span> <span lang="en-us" style="font-size:10pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us"> ratios were also found effective on fresh and dry
weights, and alizarin and purpurin contents of the samples tested. 1:2
inoculation ratio caused higher alizarin and purpurin contents in cell
suspension cultures, but biomass amounts were found higher at 1:4 inoculation
ratio. </span> <span lang="en-us" style="font-size:10pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us">The highest
alizarin ( </span> <span lang="en-us" style="font-size:10pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us">3.183 mg/g) </span> <span lang="en-us" style="font-size:10pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us">and
purpurin ( </span> <span lang="en-us" style="font-size:10pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us">1.153 mg/g) </span> <span lang="en-us" style="font-size:10pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us">contents
were detected in the sample that had 1:2 inoculation ratios and was obtained
from the medium at 200 mM NaCl concentration. </span> </p> <p> </p>}, number={3}, publisher={Sakarya University}