@article{article_285656, title={DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE)}, journal={Trakya University Journal of Natural Sciences}, volume={18}, pages={33–38}, year={2017}, DOI={10.23902/trkjnat.285656}, author={Örtücü, Serkan and Albayrak İskender, Nurcan}, keywords={Beauveria bassiana,broad host range,Tetranychus urticae,enzyme activity}, abstract={<p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:150%;"> <span lang="en-us" style="font-size:12pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us"> <span lang="en-us" style="margin:0px;line-height:115%;font-family:’Times New Roman’, serif;font-size:9pt;" xml:lang="en-us">Broad host </span> <span style="margin:0px;line-height:115%;font-family:’Times New Roman’, serif;font-size:9pt;">range </span> <span lang="en-us" style="margin:0px;line-height:115%;font-family:’Times New Roman’, serif;font-size:9pt;" xml:lang="en-us"> of entomopathogenic fungi may
have a commercial advantage for biological control industry. In addition, all
entomopathogenic fungi rely on a combination of different enzymes to penetrate
their hosts and these enzymes play an important role in the host range of
fungal isolates. The aim of this study is to determine the potential of three <i>Beauveria bassiana </i> (Bals.) Vull.
isolates in control of <i>Tetranychus
urticae </i> Koch (Acari: Tetranychidae) and to determine their lipase,
chitinase, and protease activities. For this purpose, <i>B. bassiana </i> (PaF04, PaF09 and PaF76) isolates, which was previously
demonstrated to have entomopathogenic activity against Coleoptera, Lepidoptera,
and Hymenoptera were chosen. <i>T. urticae </i>
was used as the test organisms. Three different suspensions (1x10 <sup> <font size="1">6 </font> </sup>,
1x10 <sup> <font size="1">7 </font> </sup> and 1x10 <sup> <font size="1">8 </font> </sup> conidia ml <sup> <font size="1">-1 </font> </sup>) were prepared in
Tween 80. 2ml suspension of a single dose was sprayed onto down sides of bean
leaf discs using hand sprayer. Mortality rates of <i>T. urticae </i>, in terms of LT50 values, were recorded daily for 5 days.
Enzyme activities were investigated by petri plate assays and clear zones
formed around the colonies indicated enzymatic activities. All tested fungal
isolates were pathogenic to the <i>T.
urticae </i> causing mortality between 64.2±5.6% and 100% on the 5 <sup> <font size="1">th </font> </sup>day
after the application. LT50 values varied between 3.16±0.2 and 3.72±0.5 days.
Mortality caused by PaF04 </span> <span style="margin:0px;line-height:115%;font-family:’Times New Roman’, serif;font-size:9pt;">isolate </span> <span lang="en-us" style="margin:0px;line-height:115%;font-family:’Times New Roman’, serif;font-size:9pt;" xml:lang="en-us"> was found to be significantly
different (p<0.05). In conclusion, PaF isolates of <i>B. bassiana </i> have been determined to have potential to suppress <i>T. urticae </i>, in addition to their
potential in control of Coleoptera, Lepidoptera, and Hymenoptera. The present
results showed that the isolates used, especially PaF04, have a broad host
range. </span> </span> </p>}, number={1}, publisher={Trakya University}