TY  - JOUR
TT  - Investigation And Method Optimization For The Determination Of Noscapine And Related Stress Degradation Compounds By Lıquid Chromatography Coupled To Tandem Mass Spectrometry (LCMS/MS)
AU  - Gökçe, Süleyman
AU  - Bulduk, İbrahim
AU  - Enginar, Hüseyin
PY  - 2017
DA  - January
JF  - Duzce University Journal of Science and Technology
JO  - DÜBİTED
PB  - Duzce University
WT  - DergiPark
SN  - 2148-2446
SP  - 231
EP  - 241
VL  - 5
IS  - 1
KW  - Bozunma ürünleri
KW  - LC-MS/MS
KW  - Noskapin
N2  - The tubuline-binding anticancer activity of noscapine, an orally-available plant-derived anti-tussive alcaloide, has been recently identified. Noscapine inhibits tumor growth in nude mice bearing human xenografts of hematopoietic,breast, lung, ovarian, brain and prostate origin. In this study, Noscapine is alkaloit derivative and pharmaceutical active substance. Objective was to investigate the degradation behavior of Noscapine under different recommended stress conditions according to International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) by LC-MS/MS. The forced degradation testing of Noscapine was carried out for acidic, basic, photolysis, oxidative, and thermal degradation. The degradation appeared using a reserved-phase C18 column at 40 C0 with a mobile phase comprised of methanol:H2O (50:50, v/v) with formic acid %0,1, having a flow rate of 1,2 ml min-1 and a detection wavelenght at 310 nm. The mass spectrometer was operated in the positive ion mode for detection. The precursor to product ions selected for Noscapine qualitative optimization were m/z 414.1 respectively. The LCMS/MS method was also adopted for he characterization of degradation products. Based on the m/z values and fragmentation patterns, the degradation pathway of the Noscapine has been proposed. 
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UR  - https://dergipark.org.tr/en/pub/dubited/article/289298
L1  - https://dergipark.org.tr/en/download/article-file/282415
ER  -