TY  - JOUR
TT  - Investigation of Alkaloids in Callus and Multiple Shoots Obtained from Catharantus roseus L.
AU  - Altan, Filiz
AU  - Duru, Mehmet
PY  - 2017
DA  - September
Y2  - 2017
DO  - 10.29133/yyutbd.290146
JF  - Yuzuncu Yıl University Journal of Agricultural Sciences
JO  - YYU J AGR SCI
PB  - Van Yuzuncu Yıl University
WT  - DergiPark
SN  - 1308-7576
SP  - 337
EP  - 346
VL  - 27
IS  - 3
KW  - Catharanthus roseus
KW  - Çoklu sürgün
KW  - In vitro tohum çimlenmesi
KW  - Kallus
KW  - Triptofan
N2  - In this study, the in vitro sterile plantlets and callus production in Catharanthus roseus L., which is rich in the number of alkaloid and arouses interest especially in cancer treatments, the multiple shoot production from sterile plantlets and the alkaloid analysis were made in both productions. C.roseus seeds were firstly germinated in full-power MS medium, the hypocotyl, cotyledon and root explants were promoted to callus production in different plant growth regulators supplemented basic MS medium. The dominant presence of a class of alkaloid (tryptophan) was identified in the 0. 1 mg L-1 kinetin+ 1 mg L-1 NAA and 0.1 mg L-1 kinetin+ 2 mg L-1 2.4 D supplemented nutrition environment. In the study, the plantlets (root and hypocotyl + cotyledon) germinated in 1/2 MS medium were also separated from their roots and were cultivated in basic MS medium supplemented with different concentrations of BA for promotion of multiple shoot growth. To control alkaloid analyses were made for in vivo grown C.roseus plant (provided during the flowering period). The best shoot growth was seen in 1 mg L-1 BA supplemented nutrition environment and the obtained serpentine indol alkaloid was determined to be higher than the control plant although alkaloids obtained from the shoots grown in that nutrition environment were the same compounds as the control plant. Additionally, tryptamine alkaloid occurred only in control and the shoots obtained from that nutrition environment.
CR  - Akçam E ve Yürekli AK (1995). Effect of different nutrient media and explant sources on callus induction of Catharanthus roseus L. G. Don plant. Turkish Journal of Botany, (19): 569-572.
CR  - Almagro L, Fernandez-Perez F, Pedreno MA (2015). Indole alkaloids from Catharanthus roseus: bioproduction and their effect on human helath. Molecules 20:2973-3000.
CR  - Arvy MP, Imbault N, Naudascher F, Thiersault M, Doircau P (1994). 2.4 D and alkaloid accumulation in periwinkle cell suspensions. Biochimie 76(5): 410-416.
CR  - Ataei-Azimi A, Hashemloian BD, Ebrahimzadeh H, Majd A (2008). High in vitro production of ant-canceric indole alkaloids from periwinkle (Catharanthus roseus) tissue culture. African Journal of Biotechnology 7(16): 2834-2839.
CR  - Carew DP, Krueger RJ (1977). Catharanthus roseus tissue culture: the effects of medium modifications on growth and alkaloid production. Lloydia 40(4): 326-336.
UR  - https://doi.org/10.29133/yyutbd.290146
L1  - https://dergipark.org.tr/en/download/article-file/351615
ER  -