@article{article_292276, title={Recombinant DNA Polymerase Production of Thermococcus gorgonarius}, journal={Selcuk University Journal of Science Faculty}, volume={43}, pages={1–9}, year={2017}, author={Çadırcı, Bilge Hilal and Bebek, Oğuzhan and Çam, Deniz}, keywords={DNA polymerase,PCR,Recombinant DNA technology,Thermococcus gorgonarius}, abstract={<p>A thermostable enzyme Tgo DNA polymerase was produced by a hyperthermophilic archaea, Thermococcus gorgonarius. In this study, it was aimed to produce the Tgo DNA polymerase which synthesizes highly qualified DNA by its 3’-5’-exonuclease activity, by recombinant DNA technology. For this purpose, synthesized synthetic DNA was cut with restriction enzymes and ligated into a pET28a vector. The activity of the enzyme expressed in E. coli BL21 was controlled by PCR reaction. <br /> </p>}, number={1}, publisher={Selcuk University}