TY  - JOUR
TT  - DETECTION OF FRAUDULENT PRACTICES INVOLVING SOME PLANT DERIVED COMPOUNDS IN FOODS USING REAL-TIME PCR
AU  - Kesmen, Zülal
AU  - Büyükkiraz, Mine E.
AU  - Kahraman, Neslihan
AU  - Yetim, Hasan
PY  - 2017
DA  - May
JF  - Gıda
JO  - GIDA
PB  - The Association of Food Technology
WT  - DergiPark
SN  - 1300-3070
SP  - 305
EP  - 314
VL  - 42
IS  - 3
KW  - Gıda
KW  - tağşiş
KW  - bitki türleri
KW  - gerçek zamanlı PZR
N2  - In this country, market research have revealed that there has beenadulteration in food products in which some plant species have been replacedwith plant ingredients that have a lower economic value. Fraudulent practicesinvolving pistachios, chestnuts and almonds are prominent among theseactivities. For the protection of consumers and to prevent unfair competitionin the market, the detection of fraudulent practices is required with preciseand reliable methods. In this study, a new method based on the real- time PCRTaqMan probe technique was developed to determine the fraudulent use of peainstead of pistachio, apricot kernel instead of almond and common bean insteadof chestnut in some food products. For this purpose, species-specific primerand probe sets were designed on the chloroplast tRNA-Leu (trnL) gene for pea and bean and also on the nuclear S14 F- boxprotein gene for apricot. In the real-time PCR reactions performed with thedesigned species specific primer and probe sets, apricot could be detected atthe level of 0.1%, pea at the level of 0.01% and bean at the level of 0.001%,quantitatively, without any cross-reactivity with other plant species examined.Additionally, the developed method also provided successful detection in binarymixtures of the plants: pistachio-pea, chestnut-bean and apricot kernel-almond.As a result, it was concluded that the method provided a sensitive and reliabledetection method for pea, bean and apricot kernel residues in foods, and it cansuccessfully be used in the routine control of fraudulent practices involvingthese plant species in food products.
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UR  - https://dergipark.org.tr/en/pub/gida/issue//298497
L1  - https://dergipark.org.tr/en/download/article-file/284258
ER  -