@article{article_304303, title={Genoprotective Potential of Total Anthocyanin Extracted from Prunus avium}, journal={Sakarya University Journal of Science}, volume={21}, pages={1201–1209}, year={2017}, DOI={10.16984/saufenbilder.304303}, author={Berber, Ahmet Ali and Sönmez, Fatih and Berber, Nurcan and Demir, Taki and Doğancı, Merve Ayşe and Aygün, Betül and Aksoy, Hüseyin}, keywords={Anthocyahins,Prunus avium,Antigenotoxicity,Comet,Chromosomal aberration,Micronucleus}, abstract={<p class="Abstract">Sweet cherries (Prunus avium) are rich in flavonoids such as
anthocyanins that are beneficial for the immune system.  The aim of the present study was to
investigate in vitro antigenotoxicity and anticytotoxicity of total antochyanins
extracted from Prunus avium (TAP) using by chromosomal aberration (CA), single
cell gel electrophoresis (Comet) and micronucleus assay (MN) in human
peripheral lymphocytes. 50, 100, 200 and 400 µg/mL concentrations of TAP was
screened for its protective activity using mytomicin-C and H <sub>2 </sub>O <sub>2 </sub>  induced DNA damage in human lymphocytes.
Also, positive, negative and solvent controls were used.  According to the positive control TAP
statistically decreased the abnormal cell percentage and CA/cell in 200 and 400
µg/mL at 24 h treatment and in all concentrations at 48 h. On the other hand,
it was significantly increased the mitotic index in only 200 µg/mL at 24h and
in all concentrations (except 200µg/mL) at 48h. 
Similarly, TAP were reduced MN frequency in all concentrations according
to the positive control. In the comet assay, significant decreases in comet
tail length, tail moment and tail intensity were observed in all
concentrations. According to these results, we demonstrate that TAP has potent
anticytotoxic and antigenotoxic effect against MMC and H <sub>2 </sub>O <sub>2 </sub>-
induced genotoxicity. </p> <p> </p>}, number={6}, publisher={Sakarya University}