TY  - JOUR
TT  - Prunus avium’dan ekstrakte edilen total antosiyaninlerin genoprotektif etkisi
AU  - Berber, Ahmet Ali
AU  - Sönmez, Fatih
AU  - Berber, Nurcan
AU  - Demir, Taki
AU  - Doğancı, Merve Ayşe
AU  - Aygün, Betül
AU  - Aksoy, Hüseyin
PY  - 2017
DA  - December
Y2  - 2017
DO  - 10.16984/saufenbilder.304303
JF  - Sakarya University Journal of Science
JO  - SAUJS
PB  - Sakarya University
WT  - DergiPark
SN  - 2147-835X
SP  - 1201
EP  - 1209
VL  - 21
IS  - 6
KW  - Anthocyahins
KW  - Prunus avium
KW  - Antigenotoxicity
KW  - Comet
KW  - Chromosomal aberration
KW  - Micronucleus
N2  - Kiraz (Prunus avium) antosiyanin gibi immün sistem içinfaydalı olan flavonoidler açısından oldukça zengin bir bitkidir. Bu çalışmadaamaç Prunus avium’dan ekstrakte edilen total antosiyaninlerin (TAP) in vitroantigenotoksik ve antisitotoksik etkilerini insan lenfositlerinde kromozomalanormallik (CA), tek hücre jel elektroforezi (Comet) ve Mikronükleus testleri(MN) ile değerlendirmektir. Çalışmamızda, insan lenfositlerinde mitomisin-C veH2O2 ile indüklenen DNA hasarına karşı TAP’ın 50, 100, 200and 400 µg/mL’lik konsantrasyonlarının koruyucu etkisi gözlemlenmiştir. Ayrıca, pozitif, negatif ve çözücü kontrolgrupları da dahil edilmiştir. Pozitifkontrole göre TAP; 24 saatlik uygulamanın 200 ve 400 µg/mL’lik, 48 saatlikuygulamanın ise tüm konsantrasyonlarında anormal hücre yüzdesini ve CA/hücrefrekansını istatistiksel olarak anlamlı bir şekilde azaltmıştır. Benzer şekildeTAP, mitotik indeksi istatistiksel olarak anlamlı bir şekilde 24 saatlikuygulamanın sadece 200 µg/mL’lik konsantrasyonunda artırırken, 48 saatlikuygulamanın ise tüm dozlarında (200 µg/mL’lik konsantrasyon hariç) artırmıştır.Bu sonuçlara paralel olarak TAP, MN frekansını da pozitif kontrole göre tümkonsantrasyonlarda istatistiksel olarak anlamlı bir şekilde azaltmıştır. Comettestinde yapılan TAP uygulamasıyla tüm dozlarda kuyruk uzunluğu, kuyruk momentive kuyruk yoğunluğunda da anlamlı bir azalma olduğu gözlemlenmiştir. Eldeedilen bu sonuçlara göre, TAP’ın mitomisin-C ve H2O2 gibigenotoksik ajanlara karşı potansiyel antisitotoksik ve antigenotoksik koruyucubir etkiye sahip olduğu gösterilmiştir.
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UR  - https://doi.org/10.16984/saufenbilder.304303
L1  - https://dergipark.org.tr/en/download/article-file/373960
ER  -