TY - JOUR TT - The Effect of Wastewater Sludge on Soil Enzyme Activities in Soil Contaminated with Crude Oil and Waste Engine Oil AU - Dindar, Efsun AU - Topaç Şağban, F. Olcay Topaç Şağban AU - Başkaya, Hüseyin Savaş PY - 2017 DA - April Y2 - 2017 DO - 10.17482/uumfd.305207 JF - Uludağ Üniversitesi Mühendislik Fakültesi Dergisi JO - UUJFE PB - Bursa Uludağ University WT - DergiPark SN - 2148-4155 SP - 81 EP - 94 VL - 22 IS - 1 KW - atık yağ KW - enzim aktiviteleri KW - ham petrol KW - inkübasyon KW - toprak N2 - Petroleum products cause great devastation in soil ecosystems. Thisresults from the fact that these substances are mixtures of organic compoundswith a low bioavailability, often described as potentially carcinogenic andmutagenic. As a result of the high potential of petroleum hydrocarbons toaccumulate in the soil environment, together with their resistance tobiodegradation, these compounds cause substantial changes in biologicalparameters, such as enzymatic activity and abundance of microbes. The objective of this study was toevaluate the effects of different types of hydrocarbon pollution (crude oil andwaste engine oil) on soil enzyme activities at the end of incubation period of12 months. The possible use of wastewater sludge as a biostimulating agent inpetroleum-contaminated soils was also evaluated. Enhanced enzyme activitylevels in contaminated soils indicated that crude oil and waste engine oilappeared to stimulate microbial growth and enzyme activity in the soilenvironment. Results showed that hydrocarbon contamination inhibited enzymaticactivities (except β-glucosidase) in all the amended soil samples. The effect of wastewater sludge amendment onsoil enzymes in polluted soil appears to depend on contaminant dose and theorigin of the petroleum product. CR - Achuba, F.I., Peretiemo Clarke, B.O. (2008) Effect of spent engine oil on soil catalase and dehydrogenase activities. International Agrophysics, 22, 1-4. CR - Anonim, (1985). Standard Methods for the Examination of Water and Wastewater. APHA-AWWA-WPCF, Copyright by American Public Health Association, Washington, p. 1269. CR - Boonchan, S., Britz, M.L., Stanley, G.A. (2000) Degradation and mineralization of high-Molekular-weight polycyclic Aromatic hydrocarbons by defined fungal bacterial cocultures. Applied and Environmental Microbiology. 66, 3, 1007–1019. doi: 10.1128/AEM.66.3.1007-1019.2000 CR - Bremner, JM, Mulvaney CS (1982) Nitrogen-total. In: Page, A.L., Miller, R.H. (Eds.), Methods of Soil Analysis. Part 2, Agron. Monogr, second ed., vol. 9. ASA and SSSA, Madison, WI, pp. 595-624. CR - Brohon, B., Delolme, C., Gourdon, R. (2001) Complementarity of bioassays and microbial activity measurements for the evaluation of hydrocarbon-contaminated soils quality. Soil Biology and Biochemistry, 33, 883-891. doi:10.1016/S0038-0717(00)00234-0 CR - Caravaca, F., Roldan, A. (2003) Assessing changes in physical and biological properties in a soil contaminated by oil sludges under semiarid Mediterranean condition. Geoderma. 117; 53-61. doi:10.1016/S0016-7061(03)00118-6 CR - Dick, W.A., Cheng, L., Wang, P. (2000) Soil acid and alkaline phosphatase activity as pH adjustment indicators. Soil Biology and Biochemistry, Volume 32 (13): 1915-1919. doi: 10.1016/S0038-0717(00)00166-8 CR - Dindar, E. (2008) Arıtma Çamuru Verilen Tarım Topraklarında Solucan Aktivitesinin Azot Formlarına ve Toprak Enzim Aktivitelerine Etkisi. Yüksek Lisans Tezi, U.Ü Fen Bilimleri Enstitüsü, Çevre Mühendisliği Anabilim Dalı, Bursa. CR - Dindar, E., Topaç Sağban, F.O., Alkan, U., Başkaya, H.S. (2010) The influence of wastewater sludge amendment on the nitrogen content and urease activity of soil with earthworms. Fresenius Environment Bulletin, 19, 8a, 1655-1660. CR - Dindar, E. (2014) Petrol ve Petrol Ürünleriyle Kirlenmiş Toprakların Islahı, Doktora Tezi, U.Ü Fen Bilimleri Enstitüsü, Çevre Mühendisliği Anabilim Dalı Bursa. CR - Esen, A. (2010) β-Glucosidases, overview. In: ESEN, A. (Ed), β-Glucosidase: Biochemistry and Molecular Biology. American Chemical Society, Washington, DC, pp. 1-13. CR - Frankenberger, W.T., Johanson, J.B. (1982) Influence of crude oil and refin petroleum products on soil dehydrogenase activity. J. Environ Qualtiy, 11, 602-607. doi:10.2134/jeq1982.00472425001100040010x CR - Frankenberger, W.T. (1992) The need for a laboratory feasibility study in bioremediation of petroleum hydrocarbons, In: E. J. Calabrese andP. T. Kostecki, Eds., Hydrocarbon Contaminated Soils and Groundwater, Lewis Publication, Boca Raton, 1992, pp. 237-293. CR - Isaac, R.A., Johnson, Jr W.C. (1998) Elemental determination by inductively coupled plasma atomic emission spectrometry. In: Kalra, Y.P. (Ed.), Handbook of Reference Methods for Plant Analysis. CRC Press, Boca Raton, Florida, USA., pp. 165-170. CR - Janke, S., Schamber, H., Kunze, C. (1992) Beeinflussung der bodenbiologischen Aktivitat durch Heizöl. Angew. Botanik 66, 42- 45. CR - Labud, V., Garcia, C., Hernandez, T. (2007) Effect of hydrocarbon pollution on the microbial properties of a sandy and a clay soil. Chemosphere. 66: 1863-1871. doi:10.1016/j.chemosphere.2006.08.021 CR - Li, G., Xia, X., Yang, Z., Wang, R., Voulvoulis N. (2006) Distribution and sources of polycyclic aromatic hydrocarbons in the middle and lower reaches of the Yellow River, China, Environmental Pollution, 144, 985-993. doi:10.1016/j.envpol.2006.01.047. CR - Keeney, D.R., Nelson, D.W. (1982) Nitrogen inorganic forms. In: Page, A.L. (Ed.), Methods of Soil Analysis, Part 2, Agron. Monogr, second ed., vol. 9. ASA and SSSA, Madison, WI, pp. 643-698. CR - Kızılkaya, R., Arcak, S.,Horuz, A., Karaca A. (1998) Çeltik tarımı yapılan toprakların enzim aktiviteleri üzerine toprak özelliklerinin etkisi, Pamukkale Üniversitesi, Mühendislik Bilimleri Dergisi, 4,3, 797-804. CR - Kirk, J.L., Montoglis, P., Klironomos, J., Lee, H., Trevors, J.T., (2005) Toxicity of diesel fuel to germination,growth and colonization of Glomus intraradices in soil and in vitro transformed carrot root cultures. Plant Soil 270, 23-30. CR - Kremer, R.I., Li, J. (2003) Developing weed-suppressive soils through improved soil quality management. Soil Till. Res. 72: 193-202. doi:10.1016/S0167-1987(03)00088-6 CR - Margesin, R., Zimmerbauer, A., Schinner, F. (2000a) Monitoring of bioremediation by soil biological activities. Chemosphere. Vol.40, Issue 4, p. 339-346. doi:10.1016/S0045-6535(99)00218-0 CR - Margesin, R., Walder, G., Schinner, F. (2000b) The impact of hydrocarbon remediation (diesel oil and polycyclic aromatic hydrocarbons) on enzyme activities and microbial properties of soil. Biotechnology.world congress on biotechnology, Berlin, Allemagne. Vol (20),no:3-4,p: 313-333. CR - McLean, E.O. (1982) Soil pH and lime requirement. In: Page, A.L., Miller, R.H., Keeney, D.R. (Eds.), Methods of Soil Analysis. Part 2: Chemical and Microbiological Properties, second ed. Am. Soc. Agron., Madison, WI, pp. 199-224 CR - Mohn, W.W., Stewart, G.R. (2000) Limiting factors for hydrocarbon biodegradation at low temperature in Arctic soils. Soil Biol Biochem 32(8):1161–1172. doi:10.1016/S0038-0717(00)00032-8 CR - Ndiaye, E.L., Sandeno, J.M., McGrath, D., Dick, R.P., (2000) Integrative biological indicators for detecting change in soil quality. Am. J. Alter. Agric. 15:26–36. doi:10.1017/S0889189300008432 CR - Nelson, D.W., Sommer, L.E. (1982) Total carbon, organic carbon, and organic matter. In: Page, A.L. (Ed.), Methods of Soil Analysis, ASA Monogr, second ed., vol. 9(2). American Society of Agronomy, Madison, WI, pp. 539-579. CR - Odokuma, L.O., Dickson, A.A. (2003a) Bioremediation of a crude oil polluted tropical mangrove environment. Journal of Applied Science and Environmental Management, vol 7:2, 23-29.doi: 10.4314/jasem.v7i2.17207 CR - Odokuma, L.O., Dickson, A.A. (2003b) Bioremediation of a crude oil polluted tropical rain Forest soil. Global Journal of Environmental Science. Vol:2, No:1;29-40. doi:10.4314/gjes.v2i1.2403 CR - Pascual, J.A., Hernandez T., Garcia C. ve ve Ayuso, M., (1998) Enzymatic activities in an arid soil amended with urban organic wastes: Laboratory experiment, Bioresource Technology, 64, 131-138. doi:10.1016/S0960-8524(97)00171-5 CR - Rhoades, J.D., (1982) Cation exchange capacity. In: Page, A.L., Miller, R.H., Keeney, D.R. (Eds.), Methods of Soil Analysis. Part 2: Chemical and Microbiological Properties, second ed. Am. Soc. Agron., Madison, WI, pp. 149-158. CR - Tabatabai, M.A. (1994) Soil Enzymes, Methods of Soil Analysis, Part 2. Chemical and Microbiological Properties. Agronomy Monograph No:9 (2nd ed.) ASA-SSSA. Madison, Wisconsin. USA, p. 903-943. CR - Van Hamme J. D., Singh A., Ward O. P. (2003) Recent Advances in Petroleum Microbiology, Microbiol. Mol. Biol. Rev., Cilt 67, No. 4, s.503-549. doi:10.1128/MMBR.67.4.503-549.2003 CR - Van der Waarde, J.J.V., Dijkhuis, E.L., Henssen, M.J.C. and Keuning, S. (1995) Enzyme assays as indicators for biodegradation. In van den Brinck, W. J., Bomsan, R., Arendt, F. (Eds.). Contaminated Soil’95. Kluwer Academic Publishers, Dordrecht, 1995, pp. 1377-1378. CR - Wyszkowska, J., Kucharski, J. (2004) The biochemical properties of soil contaminated by diesel oil and the yield of yellow lupin. Roez. Glebozn. 50, 299. CR - Wyszkowska, J., Kucharski, J. (2005) Correlation between the number of microorganisms and soil contamination with diesel oil . Pol. J. Environ. St. 14 (3) 359. CR - Wyszkowska, J.,. Kucharski , M., Kucharski, J. (2006) Application of the activity of soil enzymes in the Evaluation of soil contamination. Pol J Environ Stud, 3, 501–506. CR - Yılmaz M. (2012) The energy potential of Turkey and its importance of renewable energy sources in terms of electricity production, Ankara Üniversitesi Çevrebilimleri Dergisi 4(2), 33-54. UR - https://doi.org/10.17482/uumfd.305207 L1 - https://dergipark.org.tr/en/download/article-file/293628 ER -