TY  - JOUR
TT  - Aspergillus sojae Tarafından Üretilen Poligalakturonazın Kısmi Saflaştırılması için Kromatografik Bir Yaklaşım
AU  - Sen, İlknur
AU  - Mata-gomez, Marco A.
AU  - Rito-palomares, Marco
AU  - Tari, Canan
AU  - Dinç, Melike
PY  - 2017
DA  - April
DO  - 10.24323/akademik-gida.305767
JF  - Akademik Gıda
JO  - Akademik Gıda
PB  - Sidas Medya Ltd. Şti.
WT  - DergiPark
SN  - 1304-7582
SP  - 8
EP  - 16
VL  - 15
IS  - 1
KW  - Aspergillus sojae
KW  - Polygalacturonase
KW  - Maldi-TOF MS
KW  - Size exclusion chromatography
KW  - Ion exchange chromatography
N2  - Bu çalışmanın amacı, A. sojae mutantından poligalakturonazüretilmesi ve ham ekstraktın kromatografik yöntemlerle kısmisaflaştırılmasıdır. Peptitlerin konfirmasyonu için ilk basamak olarak, jeliçinde sindirilmiş sodyum-dodesil-sülfat-poliakrilamid-jel-elektroforezi(SDS-PAGE) jellerinde matriks-yardımlı lazer desorpsiyon/iyonlaştırmalı-uçuşzamanlı-kütle spektrometresi (Maldi-TOF MS) analizi yapılmıştır. Poligalakturonazüretimi için, katı-faz ve derin fermentasyonlarda üç farklı karbon kaynağıkullanılmıştır. Ham ekstrakt ilk olarak iyon değişim kromatografisi (IEXC) ilesaflaştırılmıştır ve ardından bunu boyut eleme kromatografisi izlemiştir. Derin[acı portakal kabuğu, şeker pancarı melası ve (NH4)2SO4]ve katı-faz (buğday kepeği, şeker pancarı ve HCl) fermentasyonlarından eldeedilen ham ekstraktlar yüksek seviyede poligalakturonaz enzim aktivitesi(sırasıyla 95.22 and 50.27 U/mL) göstermiştir. IEXC toplanmış fraksiyonunun(180, 200 ve 220 mM tuz fraksiyonları) boyut elemesi, en yüksek verimi (%36) vesaflaştırma katını (2.00) göstermiştir. SDS-PAGE’den elde edilen olasıpoligalakturonaz bantları jel içinde sindirilmiş ve peptit konfirmasyonu içinMaldi-TOF-MS ile analiz edilmiştir. 
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UR  - https://doi.org/10.24323/akademik-gida.305767
L1  - https://dergipark.org.tr/en/download/article-file/293401
ER  -