TY  - JOUR
TT  - HYPERICIN INDUCED CHANGES IN CYTOTOXICITY AND METABOLIC ACTIVITIES IN HT-29 ADENOCARCINOMA CELLS
AU  - Süloğlu, Aysun Kılıç
AU  - Selmanoğlu, Güldeniz
PY  - 2017
DA  - February
JF  - The Turkish Journal Of Occupational / Environmental Medicine and Safety
JO  - turjoem
PB  - Engin TUTKUN
WT  - DergiPark
SN  - 2149-4711
SP  - 192
EP  - 192
VL  - Volume 2
IS  - İssue 1 (1)
KW  - HYPERICIN INDUCED CHANGES IN CYTOTOXICITY AND METABOLIC ACTIVITIES IN HT-29 ADENOCARCINOMA CELLS
N2  - Photodynamictherapy (PDT) is an alternative way to the most common cancer treatmentmethods. The use of PDT as a cancer therapy is particularly attractive becauseof its selectivity on cancer cells. Hypericin (HYP) which is derived fromHypericum perforatum, continues to be one of the most promisingphotosensitizer, as it fascinates with its antitumor, antiviral andantidepressant properties. In this study, HT-29 (grade-1) human colonadenocarcinoma cells treated with 0.04 μM 0.08 μM or 0.15 μM HYP concentrationsand after 24 hours cells were irridated with fluorescent lamps. The effects ofHYP examined 16 and 24 hours after the activation. The effect of HYP on cellviability analysed with trypan blue staining and MTT tests. Besides, amounts ofglucose, lactate and activity of lactate dehydrogenase (LDH) were measured forthe metabolic activity. According to the results, in HT-29 cells as HYPconcentration increased cytotoxicity increased. While glucose consumption andlactate production decreased, the activity of LDH increased depending on theHYP concentration. In conclusion, this work demonstrates that HYP-mediated PDThave a critical role in leading alterations in metabolic pathways depending onthe HYP concentration and incubation time.
CR  - Aysun Kılıç SÜLOĞLU, Güldeniz SELMANOĞLU
CR  - Department of Biology, Faculty of Science, Hacettepe University, 06800, Beytepe Campus, Ankara, Turkey
UR  - https://dergipark.org.tr/en/pub/turjoem/issue//306341
L1  - https://dergipark.org.tr/en/download/article-file/294147
ER  -