@article{article_322015, title={The Protective Effect of Amino-guanidine, an Inducible Nitric Oxide Synthase Inhibitor, on Aluminium Sulphate Neuro-toxicity in the Rat (Wistar albino) Cerebellar Purkinje Cells: Stereological Study}, journal={Middle Black Sea Journal of Health Science}, volume={3}, pages={7–14}, year={2017}, DOI={10.19127/mbsjohs.322015}, author={Demirel Yılmaz, Burcu and Genç, Hayri and Baysal, Bengi and Eren, Banu}, keywords={Aluminium, Amino-guanidine, Cerebellum, Purkinje cell, Stereology}, abstract={<p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:normal;"> <b> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">Objective: </span> </b> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us"> Aluminium
(Al) is quite abundant in nature and humans are frequently exposed to Al in
daily life. Aluminium salts can exist in different forms and they may have
toxic impacts on several tissues including brain. In this study, potential
preventive effects of amino-guanidine (AG) (100 mg/kg, i.p.), an inducible
nitric oxide synthase inhibitor, on neuron damage to be created by aluminium
sulphate (3 mg/kg, i.c.v.) in cerebellar Purkinje cells were determined. </span> </p> <p> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:normal;"> <b> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">Methods: </span> </b> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us"> 24 female
Wistar albino rats were divided into 4 groups with 6 rats in each: Control (C),
Sham (S), Aluminium sulphate ( </span> <span style="font-family:’Times New Roman’, serif;">Al <sub>2 </sub>(SO <sub>4 </sub>) <sub>3 </sub> </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">), Aluminium
sulphate + Amino-guanidine ( </span> <span style="font-family:’Times New Roman’, serif;">Al <sub>2 </sub>(SO <sub>4 </sub>) <sub>3 </sub> </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">+AG). A
single aluminium sulphate (3 mg/kg) dose dissolved in 0.9% NaCl was injected
intracerebroventricularly to aluminium sulphate and aluminium sulphate +
amino-guanidine groups at the beginning of experiments. Following aluminium
sulphate injection, amino-guanidine (100 mg/kg) dissolved in distilled water was
injected to aluminium sulphate + amino-guanidine group intraperiteonally for 15
days. Nothing was administered to control group, a single dose of 0.9% (3
mg/kg, i.c.v.) sodium chloride (NaCl) was administered to sham group at the
beginning of experiments. Cerebellum tissues of the rats were removed 15 days
after treatments and they were assessed histopathologically and
stereologically. </span> </p> <p> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:normal;"> <b> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">Results: </span> </b> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">Stereological
optic fractionation method revealed cerebellar total number of Purkinje cells
as 417615±16238,8 in control group; 378650±20171,6 in Sham group;
272945±15499,5 in Aluminium sulphate group; 324581±16324,8 in Aluminium
sulphate + Amino-guanidine group. </span> </p> <p> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:normal;"> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:normal;"> <b> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">Conclusion: </span> </b> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">It was
concluded based on present findings that amino-guanidine reduced aluminium
induced Purkinje cell loss through nitric oxide synthase (NOS) inhibition.  </span> </p> <p> </p> <p> </p>}, number={3}, publisher={Ordu University}